Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins
Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins
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DOI：
10.1016/j.vaccine.2003.08.007
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发表时间：
2004-01-02
期刊：
影响因子：
5.5
通讯作者：
Finlay, BB
中科院分区：
文献类型：
--
作者：
Potter, AA;Klashinsky, S;Finlay, BB
牛是大肠杆菌 O157:H7 的重要宿主，可导致食物和水污染以及随后的人类疾病。这种病原体通过产生由 III 型分泌系统分泌的几种蛋白质（例如 Tir 和 EspA）来定殖其宿主。这些蛋白质在肠道定植中发挥作用，表明它们可能是开发疫苗以降低牛体内这种微生物水平的有用靶标。在实验挑战模型中，用大肠杆菌 O157:H7 分泌的蛋白质对牛进行疫苗接种，可显着减少粪便中脱落的细菌数量、脱落的动物数量以及脱落的持续时间。在典型饲养场环境中进行的临床试验中，牛的疫苗接种也显着降低了大肠杆菌 O157:H7 的患病率 (P = 0.04)。这一策略表明，可以通过对牛进行疫苗接种来降低大肠杆菌 O157:H7 的脱落水平，从而降低人类疾病的风险。 (C) 2003 Elsevier B.V. 保留所有权利。
Cattle are an important reservoir of Escherichia coli O157:H7 leading to contamination of food and water, and subsequent human disease. This pathogen colonizes its hosts by producing several proteins such as Tir and EspA that are secreted by a type III secretion system. These proteins play a role in colonization of the intestine, suggesting that they might be useful targets for the development of a vaccine to reduce levels of this organism in cattle. Vaccination of cattle with proteins secreted by E. coli O157:H7 significantly reduced the numbers of bacteria shed in feces, the numbers of animals that shed, and the duration of shedding in an experimental challenge model. Vaccination of cattle also significantly (P = 0.04) reduced the prevalence of E. coli O157:H7 in a clinical trial conducted in a typical feedlot setting. This strategy suggests it is possible to vaccinate cattle to decrease the level of E. coli O157:H7 shedding for the purpose of reducing the risk of human disease. (C) 2003 Elsevier B.V. All rights reserved.