The Fall of a Dogma? Unexpected High T-Cell Memory Response to Staphylococcus aureus in Humans
The Fall of a Dogma? Unexpected High T-Cell Memory Response to Staphylococcus aureus in Humans
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DOI：
10.1093/infdis/jiv128
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发表时间：
2015-09-01
影响因子：
6.4
通讯作者：
Broeker, Barbara M.
中科院分区：
文献类型：
--
作者：
Kolata, Julia B.;Kuehbandner, Iris;Broeker, Barbara M.
导论.虽然金黄色葡萄球菌是一种主要的病原体，但疫苗试验失败了。相反，针对S.金黄色葡萄球菌是常见的，这意味着免疫记忆的形成，并提出了一个大的游泳池的S。金黄色反应性辅助性T细胞为了阐明S.金黄色葡萄球菌特异性免疫记忆，在人类的T细胞反应的特点。人外周血单个核细胞（PBMC）对S.金黄色葡萄球菌抗原和频率。通过H-3-胸苷掺入法定量金黄色葡萄球菌特异性T细胞;通过流式细胞术测量细胞因子释放。金黄色葡萄球菌颗粒和胞外蛋白引起健康成人PBMC中的显著增殖。这反映了一种记忆反应，其中T细胞被单个S激活的频率很高。金黄色葡萄球菌抗原。整个S。估计金黄色葡萄球菌特异性T细胞库包含3.6%的T细胞，个体之间差异为35倍（范围，0.2%-5.7%）。当暴露于S。结论：金黄色葡萄球菌抗原刺激后，T细胞主要释放辅助性T细胞（Th）1/Th 17细胞因子。大量的S.金黄色葡萄球菌抗原反应记忆T淋巴细胞可能影响S.金黄色葡萄球菌感染。为了实现合理的疫苗设计，需要高度优先地探索自然获得的人类T细胞记忆。
Introduction. Though Staphylococcus aureus is a major pathogen, vaccine trials have failed. In contrast, class-switched antibodies specific to S. aureus are common, implying immune memory formation and suggesting a large pool of S. aureus-reactive helper T-cells.Objective. To elucidate the cellular arm of S. aureus-specific immune memory, the T-cell response in humans was characterized.Methods. The proliferative response of human peripheral blood mononuclear cells (PBMCs) to S. aureus antigens and the frequency of S. aureus-specific T-cells were quantified by H-3-thymidine incorporation; cytokine release was measured by flow cytometry.Results. Staphylococcus aureus particles and extracellular proteins elicited pronounced proliferation in PBMCs of healthy adults. This reflected a memory response with high frequencies of T-cells being activated by single S. aureus antigens. The whole S. aureus-specific T-cell pool was estimated to comprise 3.6% of T-cells with 35-fold differences between individuals (range, 0.2%-5.7%). When exposed to S. aureus antigens, the T-cells released predominantly but not solely T helper (Th) 1/Th17 cytokines.Conclusions. The large number of S. aureus antigen-reactive memory T-lymphocytes is likely to influence the course of S. aureus infection. To enable rational vaccine design, the naturally acquired human T-cell memory needs to be explored at high priority.