Trafficking pathway between plasma membrane and mitochondria via clathrin-mediated endocytosis
Trafficking pathway between plasma membrane and mitochondria via clathrin-mediated endocytosis
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通过网格蛋白介导的内吞作用在质膜和线粒体之间的运输途径
DOI：
10.1093/jmcb/mjy060
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发表时间：
2018-12-01
影响因子：
5.5
通讯作者：
Chen, Gang
中科院分区：
文献类型：
--
作者：
Wei, Zhongya;Su, Wenfeng;Chen, Gang
内吞作用是一种基本的细胞过程，它描述了一种跨越质膜进入细胞的主动运输形式。内吞途径由不同的膜隔间组成；内化的分子被输送到早期的内小体，其中一些被循环回到表面，而另一些分子被送到晚期的内小体和溶酶体进行降解。然而，人们对线粒体如何参与内吞途径知之甚少。在这里，我们报道了FM染料，膜无关的荧光脂质探针，可以通过网状蛋白介导的内吞作用直接从质膜运输到线粒体。FM染料进入线粒体使用微管依赖的主动转运，但其机制不同于经典的内吞途径。因此，这项研究揭示了一条先前未实现的从质膜到线粒体的脂质运输途径。
Endocytosis is a basic cellular process that describes a form of active transport across the plasma membrane into the cell. The endocytic pathway consists of distinct membrane compartments; internalized molecules are delivered to early endosomes, and some of them are recycled back to the surface, whereas other molecules are sent to late endosomes and lysosomes for degradation. However, little is known about how mitochondria are involved in the endocytic pathway. Here, we report that FM dyes, membrane-impermeant fluorescent lipid probes, can traffic to mitochondria directly from the plasma membrane by clathrin-mediated endocytosis. FM dye entry into mitochondria uses microtubule-dependent active transport, but the mechanism is different from the classical endocytic pathway. Hence, this study reveals a previously unrealized lipid trafficking pathway from the plasma membrane to mitochondria.