High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture
High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture
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DOI：
10.1016/j.vaccine.2006.06.083
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发表时间：
2007-01-02
期刊：
影响因子：
5.5
通讯作者：
Wu, Suh-Chin
中科院分区：
文献类型：
--
作者：
Liu, Chia-Chyi;Lian, Wei-Cheng;Wu, Suh-Chin
开发针对肠道病毒71型(EV71)感染的有效疫苗是控制该疾病的最佳手段。我们以前曾报道过，在2-1生物反应器中，使用无血清微载体Vero细胞培养，可以大规模制备低免疫原性EV71菌株[吴SC，刘春春，连文武。肠道病毒71型灭活疫苗微载体细胞培养工艺的优化。疫苗2004；22：3858--]。本工作进一步研究了在无血清微载体细胞培养中制备的两株高免疫原性毒株(EV71-075和EV71-117)的病毒生长和免疫原性。我们的结果表明，与有血清培养相比，无血清培养增加了感染后的细胞死亡率，降低了病毒的特异性生产力，但导致了较高的中和滴度。因此，无血清微载体培养是研制EV71灭活疫苗的有价值的方法。(C)2006爱思唯尔有限公司。保留所有权利。
Developing an effective vaccine against enterovirus 71 (EV71) infection provides the best means to control the disease. We have previously reported that large-scale preparation of a low immunogenic EV71 strain can be achieved using serum free microcarrier Vero cell culture in a 2-1 bioreactor [Wu SC, Liu CC, Lian WC. Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development. Vaccine 2004;22:3858-64]. This present work further investigated the virus growth and the immunogenicity of two high immunogenic strains (EV71-075 and EV71-117) prepared in serum-free microcarrier cell cultures. Our results showed that serum free culture increased cell death rate after infection, reduced the virus specific productivity, but resulted in elicitation of higher neutralizing titers in immunized mice as compared to that parallel obtained in serum-containing cultures. Therefore, serum-free microcarrier culture is a valuable process for developing inactivated EV71 vaccines. (c) 2006 Elsevier Ltd. All rights reserved.