Transcriptional control is relevant in the modulation of mosquito ferritin synthesis by iron.
Transcriptional control is relevant in the modulation of mosquito ferritin synthesis by iron.
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DOI：
10.1046/j.1432-1327.1999.00849.x
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发表时间：
1999-11
期刊：
影响因子：
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通讯作者：
D. Pham;J. Winzerling;Mark S. Dodson;John H. Law
中科院分区：
文献类型：
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作者：
D. Pham;J. Winzerling;Mark S. Dodson;John H. Law
在黄热病蚊子细胞（Aag2克隆）中，铁处理在16小时内诱导铁蛋白信息（mRNA）和蛋白（铁蛋白）增加3倍。这些数据与哺乳动物肝细胞和成纤维细胞的研究结果形成对比，后者的mRNA水平不随铁刺激而改变，但铁蛋白水平增加50倍。放线菌素D预处理Aag2细胞阻断了铁mRNA的诱导，降低了铁蛋白亚基的合成，提示铁诱导铁蛋白亚基的合成受转录调控。在Aag2细胞的细胞质提取物中也发现了一种推测的铁调节蛋白。
In yellow fever mosquito cells (Aag2 clone), iron treatment induces a threefold increase in ferritin message (fer mRNA) and protein (ferritin) by 16 h. These data contrast with work in mammalian hepatocytes and fibroblasts in which fer mRNA levels do not change with iron stimulation, but ferritin levels increase 50-fold. Pretreatment of the Aag2 cells with actinomycin D blocks induction of fer mRNA and reduces the ferritin subunit synthesis, suggesting that iron induction of ferritin subunit synthesis is subjected to transcriptional control. A putative iron-regulatory protein has also been identified in cytoplasmic extracts from Aag2 cells.