Matrix effects in the gas chromatographic-mass spectrometric determination of brominated analogues of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone.
Matrix effects in the gas chromatographic-mass spectrometric determination of brominated analogues of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone.
复制标题
气相色谱-质谱法测定 3-氯-4-(二氯甲基)-5-羟基-2(5H)-呋喃酮溴化类似物中的基质效应。
DOI：
10.1016/j.chroma.2003.12.008
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
T. Vartiainen
中科院分区：
文献类型：
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作者：
P. Rantakokko;Miljamartta Yritys;T. Vartiainen
发现强饮用水诱变剂MX的溴化类似物（3-氯-4-（二氯甲基）-5-羟基-2(5H)-呋喃酮）具有强基质诱导的色谱响应增强效应。我们评估了减少定量误差的不同方法，包括气相色谱进样系统的比较，改进样品提取物的清理，以及样品基质中校准标准的制备。在样品基质中制备标准品，用c18树脂清洗样品，使用Oasis HLB固相萃取（SPE），采用PTV无分裂进样气相色谱法，可获得最佳的定量准确度和长期性能。本方法可测定500ml水样中的MX和BMXs，定量限≤1ng/l。
Brominated analogues (BMXs) of the strong drinking water mutagen MX (3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone) were found to be subject to strong matrix induced chromatographic response enhancement effects. We evaluated different ways to reduce errors in quantification including comparison of gas chromatographic inlet systems, improved clean up of sample extracts, and preparation of calibration standards in the sample matrix. The best quantitative accuracy and long term performance were achieved when the calibration standards were prepared in sample matrix, samples were cleaned up with C18-resin in conjunction with solid phase extraction (SPE) with Oasis HLB cartridges, and gas chromatography with PTV splitless injection was used. This method enables the determination of MX and BMXs from 500ml water sample with quantitation limits of 1ng/l or less.