Numerical evaluation of temporal focusing characteristics in transparent and scattering media
Numerical evaluation of temporal focusing characteristics in transparent and scattering media
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DOI：
10.1364/oe.19.004937
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发表时间：
2011-03-14
期刊：
影响因子：
3.8
通讯作者：
Shoham, Shy
中科院分区：
文献类型：
--
作者：
Dana, Hod;Shoham, Shy
在非线性显微镜和多光子光操纵中，时间聚焦是一种简单的实现紧密的、光学分段激发的方法。时间聚焦的关键应用和优点涉及通过散射介质的传播，但是时间聚焦的逐渐加宽还没有被表征。通过结合一个详细的几何光学模型与蒙特-卡罗散射模拟，我们介绍并验证了一种模拟策略，预测在散射和非散射介质中的时间聚焦特性。使用模拟和实验的几个关键的光学几何形状的扩大的时间焦点宽度随着深度的增加在脑组织中的研究，并发现一个解析近似的依赖性，这种扩大的显微镜的参数在透明介质中。我们的研究结果表明，多光子的时间焦点有根本不同的增宽特性，在深部组织比空间焦点。(C)2011年美国光学学会
Temporal focusing is a simple approach for achieving tight, optically sectioned excitation in nonlinear microscopy and multiphoton photo-manipulation. Key applications and advantages of temporal focusing involve propagation through scattering media, but the progressive broadening of the temporal focus has not been characterized. By combining a detailed geometrical optics model with Monte-Carlo scattering simulations we introduce and validate a simulation strategy for predicting temporal focusing characteristics in scattering and non-scattering media. The broadening of the temporal focus width with increasing depth in brain tissue is studied using both simulations and experiments for several key optical geometries, and an analytical approximation is found for the dependence of this broadening on the microscope's parameters in a transparent medium. Our results indicate that a multiphoton temporal focus has radically different broadening characteristics in deep tissue than those of a spatial focus. (C) 2011 Optical Society of America