Mode of pulsatile follicle-stimulating hormone secretion in gonadal hormone-sufficient and -deficient women - A clinical research center study
Mode of pulsatile follicle-stimulating hormone secretion in gonadal hormone-sufficient and -deficient women - A clinical research center study
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DOI：
10.1210/jc.81.9.3208
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发表时间：
1996-09-01
影响因子：
5.8
通讯作者：
Evans, WS
中科院分区：
文献类型：
--
作者：
Booth, RA;Weltman, JY;Evans, WS
为了验证女性FSH至少部分是在不连续的分泌爆发中分泌的假设，以及在不同的性腺激素环境中，FSH分泌的特征会发生改变，我们通过免疫放射分析法每10分钟测量一次FSH，持续24小时，在绝经前女性的早期卵泡（EF），晚期卵泡（LF），和黄体中期（ML）的月经周期和绝经后（PM）妇女（n = 8，每组）。使用多参数去卷积评价分泌事件。FSH以间歇性方式分泌，LF（20 +/- 0.79）和PM（20 +/- 0.90）女性中检测到的分泌爆发次数（每24小时;平均值+/- SEM）大于EF（16 +/- 0.88）和ML（14 +/- 0.93）女性。FSH分泌爆发质量（毫国际单位/mL）在PM（12 +/- 1.6）中显著高于EF（1.8 +/- 0.21）、LF（3.1 +/- 1.3）或ML（0.8 +/- 0.11）妇女，主要反映了最大分泌速率的相对增加，而不是爆发半持续时间的增加。LF女性内源性FSH的估计半衰期（分钟）（155 +/-：18）短于EF（251 +/- 24）、ML（277 +/- 38）和PM（231 +/- 18）女性。交叉相关分析显示，在所有四组妇女中，连续配对的血清FSH和LH浓度之间存在强正相关。同时获得的LH浓度-时间序列的去卷积显示，在EF、LF和PM女性中，FSH和LH分泌事件在0 min滞后时间处具有统计学显著一致性（13-25%），而在ML期女性中，LH分泌爆发导致FSH分泌爆发10 min时具有统计学显著一致性。然而，由于75-87%的FSH和LH分泌脉冲是不一致的，我们推断在FSH和LH释放事件的产生中有不同的控制机制。总之，这些结果表明，FSH在女性中是在离散的分泌爆发中分泌的，FSH分泌爆发的质量和频率在表现出不同性腺激素环境的女性中不同，FSH和LH分泌爆发的发生率高于仅基于偶然性的预期，但总体一致率如此之低，以至于可能有不同的机制指导FSH和LH分泌活动的发生。
To test the hypothesis that FSH is secreted at least in part within discrete secretory bursts in women and that the characteristics of episodic FSH secretion are altered within differing gonadal hormone environments, we measured FSH by immunoradiometric assay every 10 min for 24 h in premenopausal women during the early follicular (EF), late follicular (LF), and midluteal (ML) phases of the menstrual cycle and in postmenopausal (PM) women (n = 8 in each group). Secretory events were evaluated using multiparameter deconvolution. FSH was secreted in an episodic manner, with the number of secretory bursts (per 24 h; mean +/- SEM) detected in LF (20 +/- 0.79) and PM (20 +/- 0.90) women being greater than that in EF (16 +/- 0.88) and ML (14 +/- 0.93) women. FSH secretory burst mass (milliinternational units per mL) was significantly higher in PM (12 +/- 1.6) than in EF (1.8 +/- 0.21), LF (3.1 +/- 1.3), or ML (0.8 +/- 0.11) women and primarily reflected a relative increase in the maximal secretory rate rather than increased burst half-duration. The estimated half-life (minutes) of endogenous FSH in LF women (155 +/-: 18) was shorter than those calculated in EF (251 +/- 24), ML (277 +/- 38), and PM (231 +/- 18) women. Cross-correlation analysis showed strongly positive associations between successively paired serum FSH and LH concentrations in all four groups of women. Deconvolution of simultaneously obtained LH concentration-time series revealed statistically significant concordance (13-25%) between FSH and LH secretory episodes at a lag time of 0 min in EF, LF, and PM women and when LH secretory bursts led FSH secretory bursts by 10 min in ML phase women. However, as 75-87% of FSH and LH secretory pulses were discordant, we infer the operation of distinct control mechanisms in the generation of FSH and LH release episodes.In summary, these results suggest that FSH is secreted within discrete secretory bursts in women, that the mass and frequency of FSH secretory bursts differ in women exhibiting various gonadal hormone environments, and that FSH and LH secretory bursts occur coincidentally at a higher rate than expected on the basis of chance alone, but at such a low overall rate of concordance that distinct mechanisms probably operate to direct episodic FSH and LH secretory activity.