Activation of myogenesis by the homeobox gene Lbx1 requires cell proliferation
Activation of myogenesis by the homeobox gene Lbx1 requires cell proliferation
复制标题
DOI：
10.1093/emboj/20.24.7174
复制
发表时间：
2001-12-17
期刊：
影响因子：
11.4
通讯作者：
Braun, T
中科院分区：
文献类型：
--
作者：
Mennerich, D;Braun, T
成肌分化可以由有限数量的分子启动。在这项工作中，我们分析了同源盒基因Lbx1在鸡胚胎和外植体培养中的功能。我们证明，在体内和体外Lbx1的过表达导致各种肌肉标志物的强烈激活。我们发现，细胞增殖，这是强烈刺激Lbx1和Pax3，是必需的Lbx1或Pax3依赖性肌源性激活。细胞增殖的抑制阻止了肌肉分化标志物的表达，而Pax3和Lbx1的其他推定下游靶点的激活不受影响。我们的研究结果表明，Pax3和Lbx1在肌细胞形成过程中的一个关键功能是肌细胞群的扩大。肌肉前体细胞群的生长可能增加肌源性分化的偏好，从而使肌源性细胞能够对环境线索做出反应。
Myogenic differentiation can be initiated by a limited number of molecules. In this work, we analyzed the function of the homeobox gene Lbx1 in chicken embryos and explant cultures. We demonstrate that overexpression of Lbx1 in viro and in vitro leads to a strong activation of various muscle markers. We show that cell proliferation, which is strongly stimulated by Lbx1 and Pax3, is required for Lbx1- or Pax3-dependent myogenic activation. Inhibition of cell proliferation prevents expression of muscle differentiation markers, while the activation of other putative down-stream targets of Pax3 and Lbx1 is not affected. Our findings imply that a critical function of Pax3 and Lbx1 during muscle cell formation is the enlargement of muscle cell populations. The growth of the muscle precursor cell population may increase the bias for myogenic differentiation and thus enable myogenic cells to respond to environmental cues.