Association of MCP-1 promoter polymorphism with susceptibility to nasopharyngeal carcinoma
Association of MCP-1 promoter polymorphism with susceptibility to nasopharyngeal carcinoma
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MCP-1启动子多态性与鼻咽癌易感性的关系
DOI：
10.1002/jcb.27962
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发表时间：
2018
影响因子：
4
通讯作者：
Gangqiao Zhou
中科院分区：
文献类型：
--
作者：
Yuguang Niu;Guangming Zhou;Yahui Wang;Jianing Qin;Jie Ping;Qing Zhang;Bo Wei Han;Yu Xiang Liu;Chenning Yang;Yun Zhai;Hongxing Zhang;Fuchu He;Hai Qiang Mai;Jin Xin Bei;Yuanfeng Li;Gangqiao Zhou
鼻咽癌是中国南部地区人群中的常见病，受遗传因素和环境危险因素的共同影响。单核细胞趋化蛋白-1(MCP-1)是半胱氨酸-半胱氨酸趋化因子家族的成员，在肿瘤发生发展中起重要作用。MCP-1启动子rs1024611的多态已被证明与几种癌症的风险显著相关。我们的目的是评估rs1024611在鼻咽癌易感性中的作用。采用聚合酶链式反应-限制性片段长度多态性方法，对广西中国南部人群中593例鼻咽癌患者和480例非鼻咽癌患者的rs1024611进行了基因分型。我们观察到，在加性模型和显性模型中，rs1024611的G等位基因分别与鼻咽癌的风险增加显著相关(P= 0.018和0.010，优势比 = 分别为1.25和1.41)。在按年龄、性别、国籍、吸烟和饮酒状况以及吸烟水平分层的亚组中，没有发现明显的影响差异。此外，鼻咽癌组织中MCP-1的信使RNA表达水平显著高于正常鼻咽组织，rs1024611的G等位基因与EB病毒转化的淋巴细胞中MCP-1的mRNA表达水平显著相关。综上所述，我们的研究结果提示MCP-1启动子上的rs1024611可能是鼻咽癌的危险因素。为了证实这些发现，有必要进行更大样本量的进一步研究。
Nasopharyngeal carcinoma (NPC) is prevalent among populations from southern China and is influenced by both genetic and environmental risk factors. The monocyte chemoattractant protein‐1 (MCP‐1), a member of cysteine‐cysteine chemokine family, plays critical roles in cancers. A polymorphism within theMCP‐1promoter, rs1024611, has been shown to be significantly associated with the risk of several cancers. Our purpose was to assess the role of rs1024611 in NPC susceptibility. By polymerase chain reaction‐restriction fragment length polymorphism method, we genotyped rs1024611 in 593 patients with NPC (cases) and 480 cancer‐free subjects (controls) among Guangxi population from southern China. We observed that the G allele of rs1024611 was significantly associated with the increased risk of NPC in an additive model and dominant model, respectively (P= 0.018 and 0.010, odds ratio = 1.25 and 1.41, respectively). No appreciable variation of the effects was found across the subgroups stratified by age, sex, nationality, smoking and drinking status, and smoking level. In addition, significantly higher messenger RNA (mRNA) expression level ofMCP‐1was observed in NPC tissues than that in normal nasopharyngeal tissues, and the G allele of rs1024611 was significantly associated with elevated mRNA expression level ofMCP‐1in Epstein‐Barr virus‐transformed lymphocytes. In conclusion, our findings suggested that rs1024611 at theMCP‐1promoter may be a risk factor for NPC. Further studies with larger sample size are necessary to confirm these findings.