Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis
Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis
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DOI：
10.1016/s0014-5793(00)01793-2
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发表时间：
2000-07-28
期刊：
影响因子：
3.5
通讯作者：
de la Luna, S
中科院分区：
文献类型：
--
作者：
Pucharcos, C;Estivill, X;de la Luna, S
交叉蛋白1（ITSN 1）是发动蛋白的结合伴侣，已被证明参与网格蛋白介导的内吞作用。在这里，我们报告了一个新的人类基因，ITSN2，高度相似的ITSN1的表征。ITSN 2的选择性剪接产生一个短的异构体，具有两个EH结构域，一个卷曲螺旋区和五个SH3结构域，和一个较长的异构体，含有额外的羧基结构域（DH，PH和C2结构域），表明它可以作为Rho样GTP酶的鸟嘌呤核苷酸交换因子，ITSN 2的表达分析表明它在人体组织中广泛表达。Intersectin 2亚型显示出与内吞机制的其他组分相似的亚细胞分布，并与Eps15共定位。此外，它们的过表达以及相应的ITSN 1蛋白形式抑制转铁蛋白内化。(C)2000年欧洲生物化学学会联合会。由Elsevier Science B.V.出版，版权所有。
Intersectin 1 (ITSN1) is a binding partner of dynamin that has been shown to participate in clathrin-mediated endocytosis. Here we report the characterization of a new human gene, ITSN2, highly similar to ITSN1. Alternative splicing of ITSN2 generates a short isoform with two EH domains, a coiled-coil region and five SH3 domains, and a longer isoform containing extra carboxy domains (DH, PH and C2 domains), suggesting that it could act as a guanine nucleotide exchange factor for Rho-like GTPases, ITSN2 expression analysis indicates that it is widely expressed in human tissues. Intersectin 2 isoforms show a subcellular distribution similar to other components of the endocytic machinery and co-localize with Eps15. Moreover, their overexpression, as well as the corresponding ITSN1 protein forms, inhibits transferrin internalization. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.