A NOVEL SEQUENCE COMMON TO THE CENTROMERE REGIONS OF SCHIZOSACCHAROMYCES-POMBE CHROMOSOMES
A NOVEL SEQUENCE COMMON TO THE CENTROMERE REGIONS OF SCHIZOSACCHAROMYCES-POMBE CHROMOSOMES
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DOI：
10.1093/nar/15.12.4705
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发表时间：
1987-06-25
影响因子：
14.9
通讯作者：
YANAGIDA, M
中科院分区：
文献类型：
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作者：
NAKASEKO, Y;KINOSHITA, N;YANAGIDA, M
一个约4kb的长序列(命名为dh)位于庞氏链霉菌所有三条染色体的着丝粒区域。Dh每着丝粒在染色体I和II上各有一个拷贝，在染色体III的着丝粒上有多个拷贝。核苷酸序列测定表明dhI和dhII具有高度的同源性。该序列的一部分(约300-400bp)包含短的直接重复，否则dh通常在内部是非重复的。虽然在dhll中有3个片段缺失(共821bp)和两个插入(27bp)(与dhi的同源性为80%)，但在剩下的3980bp中，dhi和dhII之间只有9个替换，同源性为99.77%。取代仅限于非重复结构域，且仅为嘧啶-嘧啶或嘌呤-嘌呤类型。讨论了二氢呋喃可能的构象作用。
An approximately 4 kb long sequence (designated dh) is located in the centromere regions of all three chromosomes ofS.pombe. There is one copy each of dh per centromere in chromosomes I and II and multiples in the centromere of chromosome III. Nucleotide sequence determination shows that dhI and dhII are highly homologous. A part of the sequence (ca. 300–400 bp) contains short direct repeats, otherwise dh is in general internally non-repetitious. Although there are three segmental deletions (total 821 bp) and two insertions (27 bp) in dhll (an 80% overall homology to dhI), there are only nine substitutions between dhI and dhII in the remaining 3980 bp, giving a 99.77% homology. The substitutions are restricted to the non-repetitious domains and are only of the pyrimidine-pyrimidine or purine-purine types. A possible conformational role of dh is discussed.