Effects of chronic apical periodontitis on the inflammatory response of the aorta in hyperlipemic rats
Effects of chronic apical periodontitis on the inflammatory response of the aorta in hyperlipemic rats
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慢性根尖周炎对大鼠主动脉炎性反应的影响
DOI：
10.1007/s00784-020-03714-6
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发表时间：
2021-01-06
影响因子：
3.4
通讯作者：
Huang, Xiaojing
中科院分区：
文献类型：
--
作者：
Chen, Shuai;Ma, Dianfu;Huang, Xiaojing
目的探讨慢性根尖周炎（CAP）对大鼠主动脉炎性反应及早期病变的影响。材料与方法14周龄SD大鼠随机分为正常饮食（ND）组、高脂饮食（HFD）组、CAP组、HFD + CAP组。大鼠在受控条件下饲养，并饲喂每组规定的饲料。14周后对所有受试者实施安乐死，以进行组织病理学分析。通过酶联免疫吸附试验和实时聚合酶链反应检测血清细胞因子，以评估主动脉基因和蛋白表达的变化。结果HFD + CAP组大鼠低密度脂蛋白胆固醇水平显著高于其他各组。通过比较，HFD和HFD + CAP组大鼠的低密度脂蛋白胆固醇水平均显著低于其他组。各组间CRP水平无显著差异。而IL-2、IL-6和IL-10水平在实验CAP大鼠中较对照组升高。HFD组大鼠MCP-1、TLR-4和NF-κ B B p65的mRNA表达水平明显高于ND组。HFD + CAP组大鼠主动脉TLR-4 mRNA表达显著高于HFD组。结论CAP介导了细胞因子的高表达，诱导了主动脉的初始炎症反应。根尖牙周炎可能通过免疫反应影响炎症细胞因子（IL-2、IL-6和IL-10）的水平，临床意义CAP可能引发全身炎症反应并影响患者的主动脉。
Objectives To study the effects of chronic apical periodontitis (CAP) on the inflammatory response and initial lesion of aorta in hyperlipemic rats.Materials and methods Sprague-Dawley (SD) rats aged 14 weeks were randomly divided into 4 experimental groups (n = 8), namely, normal diet (ND), high-fat diet (HFD), CAP, and HFD + CAP. The rats were raised under controlled conditions and fed with diet specified for each group. All subjects were euthanatized after 14 weeks for histopathological analysis. Serum cytokines were analyzed to assess changes in gene and protein expression of aorta via enzyme-linked immunosorbent assay and real-time polymerase chain reaction. Results were analyzed by one-way ANOVA.Results Low-density lipoprotein cholesterol levels in rats in HFD + CAP group were significantly higher than those in other groups. By comparison, low-density lipoprotein cholesterol levels in rats in both the HFD and HFD + CAP groups were significantly lower than those in the other groups. No significant difference among all groups was observed in terms of CRP level. However, levels of IL-2, IL-6, and IL-10 increased in the experimental CAP rats compared with the control rats. mRNA expression levels of MCP-1, TLR-4, and NF-kappa B p65 were markedly elevated in rats in the HFD group compared with those in rats in the ND group. TLR-4 mRNA expression was significantly higher in rats in the HFD + CAP group than that in rats in the HFD group.Conclusions CAP mediated the high expression of cytokines and induced the initial inflammatory response in the aorta. Apical periodontitis may affect the level of inflammatory cytokines (IL-2, IL-6, and IL-10) depending on the immune response.Clinical relevance CAP may trigger a systemic inflammatory response and affect the aorta of patients.