Microbial alkaloid staurosporine induces formation of nanometer-wide membrane tubular extensions (cytonemes, membrane tethers) in human neutrophils
Microbial alkaloid staurosporine induces formation of nanometer-wide membrane tubular extensions (cytonemes, membrane tethers) in human neutrophils
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DOI：
10.4161/cam.4.1.10314
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发表时间：
2010-01-01
影响因子：
3.2
通讯作者：
Klein, Thomas
中科院分区：
文献类型：
--
作者：
Galkina, Svetlana I.;Stadnichuk, Vladimir I.;Klein, Thomas
在目前的工作中，我们表明，微生物生物碱staurosporine（STS）和Ro 31-8220，结构相关的STS蛋白激酶C抑制剂，引起人类中性粒细胞的膜管延伸后，粘附到纤连蛋白包被的基质的发展。STS诱导的肾小管延伸将中性粒细胞在网络中相互连接，并结合血清调理细菌肠道沙门氏菌鼠伤寒沙门氏菌。STS诱导的延伸的直径在160-200 nm的范围内变化。延伸物充满细胞质并覆盖有膜，因为它们包括荧光细胞质和脂质染料。无论是蛋白激酶C抑制剂H-7和双吲哚马来酰亚胺VIII，也不是酪氨酸蛋白激酶抑制剂tyrphostin AG 82和染料木素引起这样的延伸形成。STS诱导膜小管延伸形成，促进肌动蛋白细胞骨架解聚或影响NONO合成。
In the present work, we demonstrate that microbial alkaloid staurosporine (STS) and Ro 31-8220, structurally related to STS protein kinase C inhibitor, caused development of membrane tubular extensions in human neutrophils upon adhesion to fibronectin-coated substrata. STS-induced tubular extensions interconnected neutrophils in a network and bound serum-opsonized bacteria Salmonella enterica serovar Typhimurium. The diameter of STS-induced extensions varied in the range 160-200 nm. The extensions were filled with cytoplasm and covered with membrane, as they included fluorescent cytoplasmic and lipid dyes. Neither protein kinase C inhibitors H-7 and bisindolylmaleimide VIIII, nor tyrosine protein kinase inhibitors tyrphostin AG 82 and genistein caused such extensions formation. Supposedly, STS induces membrane tubular extension formation promoting actin cytoskeleton depolymerization or affecting NONO synthesis.