Regulation of proliferation and photoreceptor differentiation in fetal human retinal cell cultures.
Regulation of proliferation and photoreceptor differentiation in fetal human retinal cell cultures.
复制标题
胎儿视网膜细胞培养物中增殖和光感受器分化的调节。
DOI：
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发表时间：
1995
影响因子：
4.4
通讯作者：
T. Reh
中科院分区：
文献类型：
--
作者：
M. Kelley;J. Turner;T. Reh
目的
研究胎儿人视网膜细胞是否可以在体外长时间维持，并确定外源性生长因子是否可以用于在这些培养物中产生大量的光感受器。
方法
将人胎儿视网膜（受孕后6至13周）解剖、分离并接种到培养威尔斯孔中。已显示影响大鼠视网膜祖细胞增殖和分化的特异性生长因子和类固醇/甲状腺激素被添加到培养基中，以确定这些因子中的任何一个是否对人类视网膜细胞具有类似的作用。
结果
人胚胎视网膜细胞在体外存活并继续增殖长达300天。在对照条件下，从100，000个细胞的初始平板中产生1500万个细胞;然而，添加表皮生长因子或碱性成纤维细胞生长因子刺激增殖并导致产生超过1亿个细胞。通过向培养基中加入视黄酸或三碘甲状腺原氨酸，诱导一定百分比的这些细胞分化为光感受器。
结论
人胚胎视网膜细胞可以在体外维持和扩增，表明这种技术可能有助于产生大量的视网膜细胞。所产生的细胞的数量和类型可以通过向培养基中添加外源因子来影响。人类视网膜细胞对生长因子和激素的反应与啮齿动物视网膜细胞对相同因子的反应相似，这表明这些因子的作用在物种间是保守的。
PURPOSE
To examine whether fetal human retinal cells can be maintained in vitro over long time periods and to determine whether exogenous growth factors can be used to generate large numbers of photoreceptors within these cultures.
METHODS
Fetal human retinas (6 to 13 weeks after conception) were dissected, dissociated, and plated into culture wells. Specific growth factors and steroid/thyroid hormones, which have been shown to influence retinal progenitor cell proliferation and differentiation in rats, were added to the culture medium to determine whether any of these factors had similar effects on human retinal cells.
RESULTS
Fetal human retinal cells survived and continued to proliferate for up to 300 days in vitro. Under control conditions, 15 million cells were generated from an initial plating of 100,000 cells; however, the addition of either epidermal growth factor or basic fibroblast growth factor stimulated proliferation and resulted in the generation of more than 100 million cells. A percentage of these cells was induced to differentiate as photoreceptors by adding either retinoic acid or triiodo-thyronine to the culture medium.
CONCLUSIONS
Fetal human retinal cells can be maintained and expanded in vitro, indicating that this technique may be useful for generating large numbers of retinal cells. The number and types of cells generated can be influenced by adding exogenous factors to the culture medium. The response of human retinal cells to growth factors and hormones is similar to the response of rodent retinal cells to the same factors, suggesting that the effects of these factors are conserved across species.
影响因子：
4.6
作者：
François Guillemot;Constance L. Cepko
通讯作者：
François Guillemot;Constance L. Cepko
影响因子：
4.4
作者：
D. L. Stenkamp;J. K. Gregory;R. Adler
通讯作者：
D. L. Stenkamp;J. K. Gregory;R. Adler