Distribution of BrdU label-retaining cells in eccrine sweat glands and comparison of the percentage of BrdU-positive cells in eccrine sweat glands and in epidermis in rats
Distribution of BrdU label-retaining cells in eccrine sweat glands and comparison of the percentage of BrdU-positive cells in eccrine sweat glands and in epidermis in rats
复制标题
大鼠小汗腺中BrdU标记保留细胞的分布及小汗腺和表皮中BrdU阳性细胞百分比的比较
DOI：
10.1007/s00403-013-1397-7
复制
发表时间：
2014-03-01
影响因子：
3
通讯作者：
Fu, Xiaobing
中科院分区：
文献类型：
--
作者：
Chen, Lu;Zhang, Mingjun;Fu, Xiaobing
溴脱氧尿苷（BrdU）通常用于检测标记保留细胞（lrc）。为了确定lrc是否存在以及lrc在分泌汗腺中的分布，将20只出生后24 h内的新生SD大鼠连续4次，间隔2 h腹腔注射50 mg/kg/次BrdU，或每天2次，间隔2 h，连续4天注射。最后一次BrdU注射6周后处死大鼠，取后足垫，固定，石蜡包埋。取5微米厚的组织切片，免疫组织化学法检测BrdU的表达。结果显示，BrdU（+）细胞在汗腺的螺旋状分泌部、螺旋状导管和直管中均有分散分布，而在表皮内导管中无分布。在分泌部分，除分泌细胞外，肌上皮细胞呈标记保留。两种BrdU注射方式（50 mg/kg/次BrdU连续4次，间隔2 h）与50 mg/kg/次BrdU连续2次，间隔2 h，连续4 d)对大鼠脚垫分泌汗腺BrdU（+）细胞的百分比无显著性差异（P < 0.05）。分泌汗腺中BrdU（+）细胞的比例（4.2 +/- A 1.3%）显著高于表皮底层（0.5 +/- A 0.1℃）（P < 0.05）。综上所述，大鼠足垫分泌汗腺中存在lrc，这些lrc可能在皮肤及其附属物的稳态中起重要作用。
Bromodeoxyuridine (BrdU) has commonly been used for detecting of label-retaining cells (LRCs). To determine if there are LRCs and the distributions of LRCs in eccrine sweat glands, 20 newborn SD rats within 24 h after birth were injected intraperitoneally with 50 mg/kg/time BrdU four consecutive times at 2-h intervals, or twice daily at 2-h intervals for four consecutive days. Six weeks after the last BrdU injection, rats were sacrificed, and the hind footpads were harvested, fixed and embedded in paraffin. Five-micrometer thickness tissue sections were cut and the expression of BrdU was detected immunohistochemically. The results showed that BrdU(+) cells were scatteredly distributed in coiled secretory part and coiled duct, as well as the straight duct, but not the intraepidermal duct of eccrine sweat glands. In secretory part, besides secretory cells, myoepithelial cells showed label retaining. The percentage of BrdU(+) cells in eccrine sweat gland of rat footpads had no significant difference between the two injection methods of BrdU (50 mg/kg/time BrdU four consecutive times at 2-h intervals vs. 50 mg/kg/time BrdU twice daily at 2-h intervals for four consecutive days) (P > 0.05). The percentage of BrdU(+) cells in eccrine sweat glands (4.2 +/- A 1.3 %) was significantly higher than that in stratum basale of epidermis (0.5 +/- A 0.1 aEuro degrees) (P < 0.05). In conclusion, there were LRCs in eccrine sweat glands of rat footpads, and these LRCs might play important roles in the homeostasis of skin and its appendages.