Binding, internalization and intracellular processing of 125I-epidermal growth factor purified by isoelectric focusing.
Binding, internalization and intracellular processing of 125I-epidermal growth factor purified by isoelectric focusing.
复制标题
通过等电聚焦纯化的 125I-表皮生长因子的结合、内化和细胞内加工。
DOI：
10.1016/0006-291x(82)91866-6
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
Finch,JS
中科院分区：
文献类型：
--
作者：
Magun,BE;Planck,SR;Matrisian,LM;Finch,JS
用~（125）碘化钠对经高效液相色谱法纯化的表皮生长因子（EGF）进行碘化反应，得到5种等电点不同的主要产物。其中一些物种与大鼠成纤维细胞结合具有不同的亲和力，但以相同的效率内化。检查的internalized 125 I标记的分子显示所有的125 I-EGF物种加工成具有更多的酸性等电点的大分子。pI为4.5的125 I-EGF种类与完整的非碘化EGF的电聚焦行为相对应。其他EGF物质可能代表碘化过程中共价修饰的分子。
When epidermal growth factor (EGF) which had been extensively purified by HPLC was subjected to iodination with sodium125iodide, 5 major species of differing isoelectric points were produced. Some of these species bound to rat fibroblasts with different affinities but were internalized with equal efficiency. Examination of the internalized125I-labelled molecules revealed processing of all the125I-EGF species to macromolecules with more acidic isoelectric points. The125I-EGF species with a pI of 4.5 corresponded in electrofocusing behavior with intact non-iodinated EGF. Other EGF species probably represented molecules which were covalently modified as a result of the iodination procedure.