Callus growth and proline accumulation in response to sorbitol and sucrose-induced osmotic stress in rice
Callus growth and proline accumulation in response to sorbitol and sucrose-induced osmotic stress in rice
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DOI：
10.1023/a:1022380827034
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发表时间：
2002-01-01
影响因子：
1.5
通讯作者：
Al-Bahrany, AM
中科院分区：
文献类型：
--
作者：
Al-Khayri, JM;Al-Bahrany, AM
研究了山梨醇和蔗糖组合诱导的渗透胁迫对水稻愈伤组织生长和脯氨酸积累的影响。去壳成熟种子，品种。Hassawi）在附加有4.52 μ M 2，4-二氯苯氧基乙酸（2，4-D）和2.32 μ M 6-糠基氨基嘌呤（激动素）的MS培养基上诱导出愈伤组织。该培养基还含有29.2、58.4、87.6和116.8 mM蔗糖与0、54.9、109.8和164.7 mM山梨醇的组合。无论糖处理如何，在约35%的培养种子中观察到愈伤组织形成。随着蔗糖浓度的增加，观察到愈伤组织质量的增加，在87.6 mM时达到最大生长。愈伤组织生长响应于54.9 mM山梨醇而增强，但在更高浓度下，它是抑制性的。最好的愈伤组织的生长上获得的培养基含有54.9 mM山梨醇与87.6 mM蔗糖相结合。随着蔗糖和山梨糖醇浓度的增加，渗透压的增加会诱导脯氨酸的积累，脯氨酸的最高浓度为5.8 μ mol g（-1）（f.m.），在与116.8mM蔗糖组合的164.7mM山梨糖醇上获得。
This study investigated the influence of osmotic stress, induced by sorbitol and sucrose combinations, on growth and proline accumulation in callus cultures of rice (Oryza sativa L.). Dehusked mature seeds, cv. Hassawi, were induced to callus on MS medium supplemented with 4.52 [muM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.32 muM 6-furfurylaminopurine (kinetin). The medium also contained 29.2, 58.4, 87.6, and 116.8 mM sucrose combined with 0, 54.9, 109.8, and 164.7 mM sorbitol. Callus formation was observed in about 35% of the cultured seeds irrespective of the sugar treatment. An increase in callus mass was observed as sucrose concentration increased reaching a maximum growth at 87.6 mM. Callus growth was enhanced in response to 54.9 mM sorbitol but at higher concentration it was inhibitory. Best callus growth was obtained on a medium containing 54.9 mM sorbitol combined with 87.6 mM sucrose. Increasing osmotic stress, as a consequence of increasing sucrose and sorbitol concentrations, induced proline accumulation and the highest concentration of proline, 5.8 mumol g(-1)(f.m.), was obtained on 164.7 mM sorbitol combined with 116.8 mM sucrose.