Effects of different dose of FTY720 on lymphocyte cell cycle arrest in cardiac transplantation model of rats
Effects of different dose of FTY720 on lymphocyte cell cycle arrest in cardiac transplantation model of rats
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DOI：
10.3109/08923971003674433
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发表时间：
2010-12-01
影响因子：
3.3
通讯作者：
Li, Feng
中科院分区：
文献类型：
--
作者：
Li, Qingyuan;Li, Feng
目的：探讨不同剂量FTY 720的免疫抑制作用和细胞周期阻滞作用。方法：采用体内对照和随机对照的方法，分别研究不同剂量FTY 720的免疫抑制作用和细胞周期阻滞作用，以及FTY 720的时间和剂量依赖性。FTY 720 10 mg/kg组较FTY 720 2 mg/kg组延长。(ii)外周血淋巴细胞平均数在给药后2 h明显下降，4 h达高峰，此后稳定维持在较低水平。淋巴细胞平均总数与FTY 720剂量呈线性关系，并随着FTY 720剂量的增加而减少。(iii)FTY 720处理组胸腺和淋巴结中G1-G 0期细胞百分比增加，FTY 720 10 mg/kg处理组比FTY 720 2 mg/kg处理组更明显。FTY 720处理组小鼠胸腺和淋巴结S期和G2-M期细胞百分比均降低，且FTY 720 10 mg/kg组的降低程度大于FTY 720 2 mg/kg组。结论：FTY 720是一种有效的免疫抑制剂，其免疫抑制作用呈时间和剂量依赖性。FTY 720的细胞周期阻滞作用与剂量有关。
Purposes: To probe into immunosuppressive effect and cell cycle arrest effect of different dose of FTY720.Method: we study immunosuppressive effect and cell cycle arrest effect of different dose of FTY720 and time and dose dependence of FTY720 with control and random method in vivo, respectively.Results: (i) survival time of graft in rat cardiac transplantation model is prolonged in group treated with FTY720. It is longer in group treated with FTY720 10 mg/kg than that in group treated with FTY720 2 mg/kg. (ii) The mean sum of lymphocyte in periphery blood decrease obviously 2 h after administration, reaches its peak 4 h after administration, and then maintain at low level stably. The mean sum of lymphocyte shows a linear relationship with dose of FTY720 and decrease with increasing of dose of FTY720. (iii) The percent of cell in G1-G0 increased in group treated with FTY720, not only in thymus but also in lymph node; this is more prevalent in the group treated with FTY720 10 mg/kg than that in group treated with FTY720 2 mg/kg. Similarly, the percent of cell in S and G2-M decreased in group treated with FTY720, not only in thymus but also in lymph node, and this decrease is more associated with group treated with FTY720 10 mg/kg than the one treated with FTY720 2 mg/kg.Conclusion: FTY720 is an effective immunosuppressant and immunosuppressive effect of FTY720 show a tendency of time and dose dependent. Effect of cell cycle arrest of FTY720 is related to dose of this new immunosuppressant.