Guidance of trunk neural crest migration requires neuropilin 2/semaphorin 3F signaling
Guidance of trunk neural crest migration requires neuropilin 2/semaphorin 3F signaling
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DOI：
10.1242/dev.02187
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发表时间：
2006-01-01
期刊：
影响因子：
4.6
通讯作者：
Bronner-Fraser, M
中科院分区：
文献类型：
--
作者：
Gammill, LS;Gonzalez, C;Bronner-Fraser, M
在脊椎动物胚胎中，神经嵴细胞只通过每个体块的前半部分迁移，而避免后半部分。我们证明神经嵴细胞表达受体神经匹素2 (Npn2)，而其排斥性配体信号蛋白3F （Sema3f）仅限于后半体。在Npn2和Sema3f突变小鼠中，神经嵴细胞失去了它们的节段迁移模式，而是作为一个统一的薄片迁移，尽管体极性本身保持不变。此外，Npn2是神经嵴细胞在体外避免Sema3f的细胞自主需要。这些数据表明，Npn2/Sema3f信号通过体体引导神经嵴迁移。有趣的是，神经嵴细胞在Npn2空区仍然凝聚成节段性排列的背根神经节，这表明节段性神经嵴迁移和周围神经系统的分割是可分离的过程。
In vertebrate embryos, neural crest cells migrate only through the anterior half of each somite while avoiding the posterior half. We demonstrate that neural crest cells express the receptor neuropilin 2 (Npn2), while its repulsive ligand semaphorin 3F (Sema3f) is restricted to the posterior-half somite. In Npn2 and Sema3f mutant mice, neural crest cells lose their segmental migration pattern and instead migrate as a uniform sheet, although somite polarity itself remains unchanged. Furthermore, Npn2 is cell autonomously required for neural crest cells to avoid Sema3f in vitro. These data show that Npn2/Sema3f signaling guides neural crest migration through the somite. Interestingly, neural crest cells still condense into segmentally arranged dorsal root ganglia in Npn2 nulls, suggesting that segmental neural crest migration and segmentation of the peripheral nervous system are separable processes.