Cytotoxicity of monoclonal antibodies to a subset of Giardia isolates.
Cytotoxicity of monoclonal antibodies to a subset of Giardia isolates.
复制标题
单克隆抗体对贾第鞭毛虫分离株亚群的细胞毒性。
DOI：
10.4049/jimmunol.136.7.2628
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发表时间：
1986
影响因子：
4.4
通讯作者：
A. Aggarwal
中科院分区：
文献类型：
--
作者：
T. Nash;A. Aggarwal
以前的研究表明，一些蓝氏贾第鞭毛虫分离株不同，可以根据其DNA带型与内切酶限制性内切酶消化后，表面抗原，排泄分泌（E-S）的产品进行分类。本研究制备了抗贾第鞭毛虫的单克隆抗体。这些McAb识别170，000道尔顿的抗原，该抗原存在于这些贾第虫的表面并作为E-S抗原释放到培养基中。这种抗原以前的特点，并发现区分贾第虫的这个亚组。此外，这些单克隆抗体仅对该亚群贾第虫有细胞毒性。立即发生固定化，并在7分钟内记录到杀伤。杀伤机制仍未知，但显示为补体非依赖性的，并且不发生Fab '。这些单克隆抗体可鉴定某些分离株，并可用于贾第虫的分型。
Previous studies showed that some Giardia lamblia isolates differ and can be categorized on the basis of their DNA banding patterns after digestion with endonuclease restriction enzymes, surface antigens, and excretory-secretory (E-S) products. In the present study, monoclonal antibodies (McAb) were produced that reacted with one specific group of Giardia isolates. These McAb recognized a 170,000 dalton antigen, which was present on the surface of these Giardia and released into the medium as an E-S antigen. This antigen was previously characterized and found to distinguish this subgroup of Giardia. In addition, these McAb were cytotoxic only for this subgroup of Giardia. Immobilization occurred immediately, and killing was documented by 7 min. The mechanism(s) of killing remains unknown but was shown to be complement independent and did not occur with Fab'. These McAb identifies certain isolates and can be used to type Giardia.