SWITCHING CAPACITY OF FC-EPSILON-RII-POSITIVE AND FC-EPSILON-RII-NEGATIVE MURINE B-CELLS
SWITCHING CAPACITY OF FC-EPSILON-RII-POSITIVE AND FC-EPSILON-RII-NEGATIVE MURINE B-CELLS
复制标题
DOI：
10.1002/eji.1830231225
复制
发表时间：
1993-12-01
影响因子：
5.4
通讯作者：
WALDSCHMIDT, TJ
中科院分区：
文献类型：
--
作者：
FOY, TM;WALDSCHMIDT, TJ
在以前的研究中，我们的实验室证明了低亲和力IgE Fc受体(FcepsilonRII)在描述一些小鼠B细胞亚群方面的应用。在脾中，FcepsilonRII在成熟的常规B细胞上表达，但在边缘带B细胞上缺失。在腹膜腔中，该受体存在于所有常规B细胞上，但不表达在新鲜的腹膜Ly1/姐妹B细胞上。这份报告中的研究比较了这些B细胞对同型转换的能力。用脂多糖(LPS)和白介素4(IL-4)驱动系统，从腹膜和脾中分选纯化的FcepsilonRII阳性和阴性B细胞，检测其转化为IgG1、IgE和IgA的能力。结果表明，无论来源如何，FcepsilonRII+B细胞都能产生显著水平的IgG1和IgE。取自脾的FcepsilonRII-(边缘带)B细胞也得到了类似的结果。相比之下，FcepsilonRII-(Ly1/SISTER)腹膜B细胞能产生IgG1和IgA，但不能分泌显著水平的IgE。进一步的研究测试了内毒素和IL-4诱导的FcepsilonRII和Thy1在不同的B细胞群上的表达。这些实验证明了FcepsilonRII对所有B细胞的诱导作用，无论它们最初的静止水平如何。此外，发现Thy1仅在那些能够产生IgE的B细胞亚群上被诱导。综上所述，这些结果表明，IgE分泌和Thy1表达之间存在相关性，而FcepsilonRII的存在与同型承诺之间没有明显的相关性。
In previous studies, our laboratory demonstrated the utility of the low affinity IgE Fc receptor (FcepsilonRII) in delineating a number of murine B cell subsets. In the spleen, the FcepsilonRII is expressed on mature conventional B cells but is absent on marginal zone B cells. In the peritoneal cavity, the receptor is present on all conventional B cells, but is not expressed on fresh peritoneal Ly1/sister B cells. The studies in this report compared the ability of these B cell poputations to isotype switch. Using a lipopolysaccharide (LPS)- and interleukin (IL)-4-driven system, sort-purified FcepsilonRII- positive and -negative B cells from peritoneum and spleen were tested for switching to IgG1, IgE, and IgA. The results demonstrated that regardless of their source, FcepsilonRII+ B cells produced significant levels of IgG1 and IgE. Similar results were obtained with FcepsilonRII- (marginal zone) B cells obtained from spleen. In contrast, FcepsilonRII- (Ly1/sister) peritoneal B cells were found to produce IgG1 and IgA, but were incapable, of secreting significant levels of IgE. Further studies tested for LPS and IL-4-induced expression of FcepsilonRII and Thy1 on the various B cell populations. These experiments demonstrated the induction of the FcepsilonRII on all B cells, regardless of their initial resting levels. Additionally, Thy1 was found to be induced only on those B cell subsets capable of producing IgE. Taken together, the results demonstrate a correlation between IgE secretion and Thy1 expression, and no apparent correlation between the presence of the FcepsilonRII and isotype commitment.