Mitotic functions for SNAP45, a subunit of the small nuclear RNA-activating protein complex SNAPc.
Mitotic functions for SNAP45, a subunit of the small nuclear RNA-activating protein complex SNAPc.
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SNAP45 的有丝分裂功能，SNAP45 是小核 RNA 激活蛋白复合物 SNAPc 的一个亚基。
DOI：
10.1074/jbc.m800833200
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发表时间：
2008-05-23
期刊：
影响因子：
--
通讯作者：
Hernandez N
中科院分区：
文献类型：
--
作者：
Shanmugam M;Hernandez N
The small nuclear RNA-activating protein complex SNAPc is required for transcription of small nuclear RNA genes and binds to a proximal sequence element in their promoters. SNAPc contains five types of subunits stably associated with each other. Here we show that one of these polypeptides, SNAP45, also known as PTF δ, localizes to centrosomes during parts of mitosis, as well as to the spindle midzone during anaphase and the mid-body during telophase. Consistent with localization to these mitotic structures, both down- and up-regulation of SNAP45 lead to a G2/M arrest with cells displaying abnormal mitotic structures. In contrast, down-regulation of SNAP190, another SNAPc subunit, leads to an accumulation of cells with a G0/G1 DNA content. These results are consistent with the proposal that SNAP45 plays two roles in the cell, one as a subunit of the transcription factor SNAPc and another as a factor required for proper mitotic progression.
