Suppression of a NAC-Like Transcription Factor Gene Improves Boron-Toxicity Tolerance in Rice
Suppression of a NAC-Like Transcription Factor Gene Improves Boron-Toxicity Tolerance in Rice
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DOI：
10.1104/pp.110.171470
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发表时间：
2011-07-01
期刊：
影响因子：
7.4
通讯作者：
Matoh, Toru
中科院分区：
文献类型：
--
作者：
Ochiai, Kumiko;Shimizu, Akifumi;Matoh, Toru
我们鉴定了一个负责水稻 (Oryza sativa) 硼 (B) 毒性耐受性的基因，命名为 BORON EXCESS TOLERANT1。使用源自对 B 毒性敏感的籼稻生态型品种 IR36 和耐受性粳稻生态型品种 Nekken 1 的重组自交系，将负责耐受 B 毒性的区域缩小到 4 号染色体上的 49 kb。该区域注释了 8 个基因。通过eco-TILLING分析对B毒性敏感粳稻品种Wataribune和B毒性耐受粳稻品种Nipponbare之间的该区域的DNA序列进行比较，发现基因Os04g0477300的开放阅读框序列中存在一碱基插入突变。该基因编码 NAC（NAM、ATAF 和 CUC）样转录因子，并且该转录因子的功能在 B 毒性耐受品种中被消除。通过 RNA 干扰消除 Os04g0477300 表达的转基因植物获得了对 B 毒性的耐受性。
We identified a gene responsible for tolerance to boron (B) toxicity in rice (Oryza sativa), named BORON EXCESS TOLERANT1. Using recombinant inbred lines derived from the B-toxicity-sensitive indica-ecotype cultivar IR36 and the tolerant japonica-ecotype cultivar Nekken 1, the region responsible for tolerance to B toxicity was narrowed to 49 kb on chromosome 4. Eight genes are annotated in this region. The DNA sequence in this region was compared between the B-toxicity-sensitive japonica cultivar Wataribune and the B-toxicity-tolerant japonica cultivar Nipponbare by eco-TILLING analysis and revealed a one-base insertion mutation in the open reading frame sequence of the gene Os04g0477300. The gene encodes a NAC (NAM, ATAF, and CUC)-like transcription factor and the function of the transcript is abolished in B-toxicity-tolerant cultivars. Transgenic plants in which the expression of Os04g0477300 is abolished by RNA interference gain tolerance to B toxicity.