The mechanism of transcription termination by RNA polymerase I.
The mechanism of transcription termination by RNA polymerase I.
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RNA聚合酶I转录终止的机制。
DOI：
10.1111/j.1365-2958.1994.tb00989.x
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发表时间：
1994
影响因子：
3.6
通讯作者：
Lang,W
中科院分区：
文献类型：
--
作者：
Reeder,RH;Lang,W
真核核糖体基因转录单元的 3’ 端以短 DNA 序列为界，这些短 DNA 序列指定 RNA 聚合酶 I (poll) 的位点特异性终止。从酵母到哺乳动物的轮询终止子似乎都遵循相似的规则：它们包含一个序列特异性 DNA 结合蛋白的位点；它们只在一个方向上发挥作用； 3’端形成于结合位点的上游； 5'端侧翼序列影响3'端形成的位置和效率。最近在理解其他聚合酶引起的 RNA 链延长机制方面取得的进展提出了一种轮询终止模型，其中终止被视为聚合酶遇到暂停位点时可能出现的几种结果之一。
Eukaryotic ribosomal gene transcription units are bordered at their 3’ends by short DNA sequences which specify site‐specific termination by RNA polymerase I (poll). Poll terminators from yeast through to mammals appear to follow similar rules: they contain a site for a sequence‐specific DNA‐binding protein; they function only in one orientation; 3’ends are formed upstream of the binding site; and 5’flanking sequences influence the position and efficiency of 3’end formation. Recent progress in understanding the mechanism of RNA chain elongation by other polymerases suggests a model for poll termination in which termination is seen as one of the several outcomes possible when a polymerase encounters a pause site.