Detection of dengue viruses in field caught male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by type-specific PCR
Detection of dengue viruses in field caught male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by type-specific PCR
复制标题
DOI：
10.1603/0022-2585-38.4.475
复制
发表时间：
2001-07-01
影响因子：
2.1
通讯作者：
Yin, PF
中科院分区：
文献类型：
--
作者：
Kow, CY;Koon, LL;Yin, PF
野外雄性埃及伊蚊（Aedes aegypti（L.）用登革病毒（DEN-1、DEN-2、DEN-3、DEN-4）对固定监测点采集的20只白纹伊蚊（Aedes albopictus（Skuse））成虫进行登革病毒（DEN-1、DEN-2、DEN-3、DEN-4）检测，连续1年。使用单步逆转录（或转录酶）聚合酶链反应（PCR）（RT-PCR），然后通过半巢式PCR使用上游的一致性引物和四个类型特异性引物内的非结构蛋白3基因（NS 3）的登革病毒进行测定。DEN-1、DEN-2、DEN-3和DEN-4血清型的诊断片段的大小分别为169、362、265和426 Lp。结果表明，在新加坡，埃及伊蚊和白纹伊蚊成年雄蚊中登革热病毒阳性率分别为1.33%和2.15%。埃及伊蚊血清型以DEN-1型为主（44%），其次为DEN-2型（22.2%）、DEN-3型（22.2%）和DEN-4型（11.1%）。雄性白纹伊蚊的血清型为DEN- 4（38.9%），其次为DEN-2（33.3%）、DEN-3（16.7%）和DEN-1（11.1%）。
Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (NS3) of dengue viruses. The diagnostic fragments for DEN-1, DEN-2, DEN-3, and DEN-4 serotypes were of sizes 169, 362, 265, and 426 Lp, respectively. Results showed that in Singapore 1.33% and 2.15% of Aedes aegypti and Aedes albopictus adult male mosquitoes, respectively, were positive for dengue viruses. The serotypes detected in male Ac, aegypti was DEN-1 (44%), followed by DEN-2 (22.2%) and DEN-3 (22.2%), and DEN-4 (11.1%). For Aedes albopictus males, the serotype was DEN- 4 (38.9%), followed by DEN-2 (33.3%), DEN-3 (16.7%), and DEN-1 (11.1%).