Northern blot and in situ hybridization analyses for the neurogranin mRNA in the developing monkey cerebral cortex
Northern blot and in situ hybridization analyses for the neurogranin mRNA in the developing monkey cerebral cortex
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DOI：
10.1016/j.brainres.2006.01.062
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发表时间：
2006-03-17
期刊：
影响因子：
2.9
通讯作者：
Hayashi, M
中科院分区：
文献类型：
--
作者：
Higo, N;Oishi, T;Hayashi, M
神经颗粒素是蛋白激酶C的突触后底物，其表达与树突棘发育和突触后可塑性有关。采用原位杂交和Northem印迹技术，研究了猴大脑皮层神经颗粒素表达的发育性变化。在所检查的四个新皮层区域中的每一个中，即，前额区（von Bonin和Bailey的FD区）、颞叶联合区（TE）、初级躯体感觉区（PB）和初级视觉区（OC），北方印迹分析显示，在产前和围产期直至出生后第8天，神经颗粒素mRNA的量较低。在出生后的发育过程中，其含量逐渐增加，并在出生后70天（OC区）或出生后6个月（FD、TE和PB区）达到峰值。此后，大脑新皮层中的神经颗粒素mRNA的量逐渐减少，直到出生后2-3年。原位杂交实验也表明，在出生后70天至出生后6个月的新皮层神经颗粒素mRNA的瞬时增加。在FD区和TE区的第II层和第III层; PB区的第III层深处;以及OC区的第II层、第III层和第IV层中，瞬时增加显著。在海马，在新皮层的结果相反，神经颗粒素mRNA的表达几乎连续下降，在出生后的时期。神经颗粒素在出生后新皮层的瞬时表达增加可能是可能来自皮层下结构的传入输入的突触后修饰的分子基础。(c)2006 Elsevier B. V.保留所有权利。
Neurogranin is a postsynaptic substrate for protein kinase C, and its expression is related to dendritic spine development and postsynaptic plasticity. Using both Nor-them blot analysis and in situ hybridization techniques, we investigated the developmental changes of neurogranin expression in the monkey cerebral cortex. in each of four neocortical areas examined, i.e., the prefrontal area (area FD of von Bonin and Bailey), the temporal association area (TE), the primary somatosensory area (PB), and the primary visual area (OC), the Northern blot analysis showed that the amount of neurogranin mRNA was low during the prenatal and perinatal periods until postnatal day 8. it increased during postnatal development and reached its peak value at postnatal day 70 (in area OC) or postnatal month 6 (in area FD, TE, and PB). After that, the amount of neurogranin mRNA in the cerebral neocortex decreased gradually until postnatal years 2-3. The in situ hybridization experiments also showed a transient increase of neurogranin mRNA in the neocortex during postnatal day 70 to postnatal month 6. The transient increase was prominent in layers II and III of areas FD and TE; deep in layer III of area PB; and in layers II, III, and IV of area OC. In the hippocampus, in contrast to the results in the neocortex, the expression of neurogranin mRNA was decreased almost continuously during the postnatal period. The transiently increased expression of neurogranin in the postnatal neocortex may be a molecular basis for the postsynaptic modification of afferent inputs possibly from subcortical structures. (c) 2006 Elsevier B.V. All rights reserved.