The mitochondrial genome of the butterfly Papilio xuthus (Lepidoptera: Papilionidae) and related phylogenetic analyses
The mitochondrial genome of the butterfly Papilio xuthus (Lepidoptera: Papilionidae) and related phylogenetic analyses
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DOI：
10.1007/s11033-010-0044-z
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发表时间：
2010-12-01
影响因子：
2.8
通讯作者：
Jiang, Guo-Fang
中科院分区：
文献类型：
--
作者：
Feng, Xia;Liu, Dian-Feng;Jiang, Guo-Fang
对凤蝶（Papilio xuthus）的线粒体基因组进行了全序列测定，其核苷酸序列为13，964 bp。基因组具有与其他鳞翅目物种相同的典型基因顺序。除了tRNA（Ser）（AGN）的二氢尿嘧啶臂（DHU臂）不能形成稳定的茎-环结构外，其余tRNA均表现出与其它昆虫相同的典型三叶草结构。cox 1基因的起始密码子异常，其中ATTACG六核苷酸被认为参与了起始信号传导。利用12个线粒体蛋白质编码基因序列数据推断昆虫目之间的系统发育关系。尽管完整线粒体基因组所代表的昆虫目的数量仍然有限，但在完整序列的系统发育分析中，几个建立良好的关系是显而易见的。本文不支持同变态的单系性。系统发育分析的可利用的物种的锥虫总科，Pyraloidea，凤蝶总科和Tortricidea支持目前的形态学为基础的假设，即锥虫总科，Pyraloidea和凤蝶总科是单系（Obtectomera）。凤蝶总科（Coreanaraphaelis）和凤蝶总科（P. xuthus）组成姐妹群。
The nearly complete mitochondrial genome of the butterfly Papilio xuthus (Lepidoptera: Papilionidae) was sequenced for its nucleotide sequence of 13,964 bp. The genome has a typical gene order identical to other lepidopteran species. All tRNAs showed same stable canonical clover-leaf structure as those of other insects, except for tRNA(Ser) (AGN), in which the dihydrouracil arm (DHU arm) could not form stable stem-loop structure. Anomalous initiation codons have been observed for the cox1 gene, where the ATTACG hexa-nucleotide was believed to be involved in the initiation signaling. Twelve mitochondrial protein-coding gene sequence data were used to infer the phylogenetic relationships among the insect orders. Even though the number of insect orders represented by complete mitochondrial genomes is still limited, several well-established relationships are evident in the phylogenetic analysis of the complete sequences. Monophyly of the Homometabola was not supported in this paper. Phylogenetic analyses of the available species of Bombycoidea, Pyraloidea, Papilionoidea and Tortricidea bolstered the current morphology-based hypothesis that Bombycoidea, Pyraloidea and Papilionoidea are monophyletic (Obtectomera). Bombycoidea (Bombyx mandarina and Antheraea pernyi) and Papilionoidea (P. xuthus and Coreana raphaelis) formed a sister group.