Adsorbing/dissolving Lyoprotectant Matrix Technology for Non-cryogenic Storage of Archival Human Sera.
Adsorbing/dissolving Lyoprotectant Matrix Technology for Non-cryogenic Storage of Archival Human Sera.
复制标题
用于非低温储存人类血清档案的吸附/溶解冻干保护剂基质技术。
DOI：
10.1038/srep24186
复制
发表时间：
2016
影响因子：
4.6
通讯作者：
Aksan,Alptekin
中科院分区：
文献类型：
--
作者：
Solivio,MorwenaJ;Less,Rebekah;Rynes,MathewL;Kramer,Marcus;Aksan,Alptekin
尽管在癌症生物标志物的发现和验证方面进行了大量的研究，但迄今为止，只有不到24种生物标志物被FDA批准用于临床。一个主要原因是在生物标志物研究中无意中使用了低质量的生物标本。大多数蛋白质生物标志物极易受到收集、处理和储存等分析前因素的影响。例如，低温储存对生物标本施加非常苛刻的化学、物理和机械应力，显著影响样品质量。在这篇通讯中，我们报告了一种用于非低温稳定和储存液体生物标本的静电纺lyoprotectant基质和等温玻璃化方法的发展。冻干保护剂基质主要由海藻糖和葡聚糖（以及针对不同损伤机制的各种低浓度辅料）组成，并经过设计以减少玻璃化过程中的异质性。该技术使用五种生物标志物进行了验证；LDH， CRP， PSA， MMP-7和C3a。补液后LDH、CRP和PSA水平完全恢复，MMP-7和C3a的回收率超过90%，表明等温玻璃化冷冻是一种安全、高效、低成本的低温储存替代方法。
Despite abundant research conducted on cancer biomarker discovery and validation, to date, less than two-dozen biomarkers have been approved by the FDA for clinical use. One main reason is attributed to inadvertent use of low quality biospecimens in biomarker research. Most proteinaceous biomarkers are extremely susceptible to pre-analytical factors such as collection, processing and storage. For example, cryogenic storage imposes very harsh chemical, physical and mechanical stresses on biospecimens, significantly compromising sample quality. In this communication, we report the development of an electrospun lyoprotectant matrix and isothermal vitrification methodology for non-cryogenic stabilization and storage of liquid biospecimens. The lyoprotectant matrix was mainly composed of trehalose and dextran (and various low concentration excipients targeting different mechanisms of damage) and it was engineered to minimize heterogeneity during vitrification. The technology was validated using five biomarkers; LDH, CRP, PSA, MMP-7 and C3a. Complete recovery of LDH, CRP and PSA levels was achieved post-rehydration while more than 90% recovery was accomplished for MMP-7 and C3a, showing promise for isothermal vitrification as a safe, efficient and low-cost alternative to cryogenic storage.