Optimization of an ex vivo wound healing model in the adult human skin: Functional evaluation using photodynamic therapy
Optimization of an ex vivo wound healing model in the adult human skin: Functional evaluation using photodynamic therapy
复制标题
DOI：
10.1111/wrr.12325
复制
发表时间：
2015-09-01
影响因子：
2.9
通讯作者：
Bayat, Ardeshir
中科院分区：
文献类型：
--
作者：
Mendoza-Garcia, Jenifer;Sebastian, Anil;Bayat, Ardeshir
体外试验和人体修复动物模型的有限效用，产生了对能够进行功能测试的皮肤愈合替代模型的需求。成人皮肤伤口愈合器官培养（WHOC）为研究伤口修复和评价光动力治疗（PDT）等治疗方法提供了一个有用的模型。因此，本文的目的是确定最佳的WHOC模型，并评估PDT在修复中的作用。在WHOC模型中研究了伤口几何形状、支撑系统、生长介质、细胞和基质生物标志物。随后，通过细胞活性、细胞外基质重塑、氧化应激以及伤口修复制造商的基因和蛋白质水平来测量PDT对优化后的WHOC的影响。嵌入胶原并补充DMEM的WHOCs组织较好，表皮呈层状，真皮致密，新表皮发育。pdt后，前移的再上皮化舌长3.5倍，增殖能力强，CK-14 + p16增加(p
Limited utility of in vitro tests and animal models of human repair, create a demand for alternative models of cutaneous healing capable of functional testing. The adult human skin Wound Healing Organ Culture (WHOC) provides a useful model, to study repair and enable evaluation of therapies such as the photodynamic therapy (PDT). Thus, the aim here was to identify the optimal WHOC model and to evaluate the role of PDT in repair. Wound geometry, system of support, and growth media, cellular and matrix biomarkers were investigated in WHOC models. Subsequently, cellular activity, extracellular matrix remodeling, and oxidative stress plus gene and protein levels of makers of wound repair measured the effect of PDT on the optimized WHOC. WHOCs embedded in collagen and supplemented DMEM were better organized showing stratified epidermis and compact dermis with developing neo-epidermis. Post-PDT, the advancing reepithelialization tongue was 3.5 folds longer, and was highly proliferative with CK-14 plus p16 increased (p