High-Density Noncovalent Functionalization of DNA by Electrostatic Interactions
High-Density Noncovalent Functionalization of DNA by Electrostatic Interactions
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DOI：
10.1021/jacs.5b05432
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发表时间：
2015-10-14
影响因子：
15
通讯作者：
Herrmann, Andreas
中科院分区：
文献类型：
--
作者：
Chen, Wei;Gerasimov, Jennifer Y.;Herrmann, Andreas
有朝一日，在有机溶剂中保存DNA杂交可能会大大扩展基于DNA的技术的适用性。在这里，我们提出了一种方法，可以用来在高浓度的有机介质中溶解双链DNA。这种方法需要首先用苯胺衍生物从水环境中沉淀DNA分子，然后在有机溶剂中将该部分与含胺的表面活性剂交换。我们证明，这种方法产生了表面活性剂的完全交换，并允许用疏水的伯胺、仲胺和叔胺以及有序的功能液晶系统来修饰DNA。利用这种方法，我们制造了一种传统方法无法实现的多色光采集系统。此外，这种方法使使用小的亲水分子在有机溶剂中溶解DNA成为可能，这减少了大分子周围的屏蔽，更容易进行进一步的化学修饰。我们相信，这一方法将有助于扩大基于DNA的纳米和生物技术的范围。DNA，并使极大的
Preserving DNA hybridization in organic solvents could someday serve to significantly extend the applicability of DNA-based technologies. Here, we present a method that can be used to solubilize double-stranded DNA at high concentrations in organic media. This method requires first precipitating a DNA molecule from the aqueous environment with an anilinium derivative and subsequently exchanging this moiety with an amine-containing surfactant in organic solvent. We demonstrate that this method yields complete exchange of the surfactant and allows for the modification of DNA with hydrophobic primary, secondary, and tertiary alkylamines and ordered functional lcsystems. Using this approach, we fabricate a multichromophoric light harvesting system that would be unattainable by traditional methods. Additionally, this method makes it possible to use small, hydrophilic molecules to solubilize DNA in organic solvents, which reduces the shielding around the macromolecule more accessible for further chemical modification. We believe that this approach will prove beneficial in expanding the scope of DNA-based nano- and biotechnologies. DNA and makes the tremendously