Low Dosed Curcumin Combined with Visible Light Exposure Inhibits Renal Cell Carcinoma Metastatic Behavior in Vitros
Low Dosed Curcumin Combined with Visible Light Exposure Inhibits Renal Cell Carcinoma Metastatic Behavior in Vitros
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DOI：
10.3390/cancers12020302
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发表时间：
2020-02-01
期刊：
影响因子：
5.2
通讯作者：
Blaheta, Roman A.
中科院分区：
文献类型：
--
作者：
Rutz, Jochen;Maxeiner, Sebastian;Blaheta, Roman A.
最近的文献表明，可见光可以放大姜黄素诱导的肾细胞癌（RCC）细胞的生长停滞。本研究旨在研究这种策略是否也有助于阻断RCC的转移进展。将低剂量姜黄素（0.2 μ g/mL; 0.54 μ M）应用于A498、Caki 1或KTCTL-26细胞1小时，随后暴露于可见光5分钟（400-550 nm，5500 lx）。然后评价对人血管内皮细胞或固定化胶原的粘附。还研究了姜黄素对趋化性和迁移的影响，以及姜黄素诱导的α和β整合素表达的改变。姜黄素没有光暴露或光暴露没有姜黄素诱导没有改变，而姜黄素加光显着抑制RCC粘附，迁移和趋化性。这与所有细胞系中α 3、α 5、β 1和β 3整联蛋白的明显减少有关。单独阻断这些整合素亚型中的每一种导致肿瘤细胞粘附和趋化行为的显著改变。将低剂量姜黄素与光结合显著抑制RCC结合活性和趋化运动，并与降低的整合素α和β亚型相关。因此，姜黄素结合可见光有望抑制RCC的转移过程。
Recent documentation shows that a curcumin-induced growth arrest of renal cell carcinoma (RCC) cells can be amplified by visible light. This study was designed to investigate whether this strategy may also contribute to blocking metastatic progression of RCC. Low dosed curcumin (0.2 mu g/mL; 0.54 mu M) was applied to A498, Caki1, or KTCTL-26 cells for 1 h, followed by exposure to visible light for 5 min (400-550 nm, 5500 lx). Adhesion to human vascular endothelial cells or immobilized collagen was then evaluated. The influence of curcumin on chemotaxis and migration was also investigated, as well as curcumin induced alterations of alpha and beta integrin expression. Curcumin without light exposure or light exposure without curcumin induced no alterations, whereas curcumin plus light significantly inhibited RCC adhesion, migration, and chemotaxis. This was associated with a distinct reduction of alpha 3, alpha 5, beta 1, and beta 3 integrins in all cell lines. Separate blocking of each of these integrin subtypes led to significant modification of tumor cell adhesion and chemotactic behavior. Combining low dosed curcumin with light considerably suppressed RCC binding activity and chemotactic movement and was associated with lowered integrin alpha and beta subtypes. Therefore, curcumin combined with visible light holds promise for inhibiting metastatic processes in RCC.