Dirofilaria immitis: studies on anti-microfilarial immunity in Lewis rats.
Dirofilaria immitis: studies on anti-microfilarial immunity in Lewis rats.
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恶丝虫：Lewis 大鼠抗微丝虫免疫的研究。
DOI：
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发表时间：
1989
影响因子：
3.3
通讯作者：
A. Scott
中科院分区：
文献类型：
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作者：
W. K. Tamashiro;M. S. Ibrahim;D. Moraga;A. Scott
研究了免疫接种对雄性Lewis大鼠微丝虫清除率的影响。用全微丝、死微丝或含有弗氏佐剂的微丝PBS提取物免疫动物。免疫动物，以及未经治疗和佐剂对照，静脉注射4 × 10(5)个活微丝蚴（mf）。接种全mf的大鼠微丝虫病持续时间为15.5天，接种PBS提取物的大鼠为17.7天，单独接种佐剂的大鼠为36.3天，未接种的大鼠为70天以上。ELISA和Western blots对抗微丝虫IgG反应的分析表明，免疫诱导了大量针对高分子量肽的抗体，特别是位于105 kDa的肽。两组免疫动物的抗体水平在攻毒后继续升高，在清除微丝虫当天达到78-80微克/毫升的峰值水平。攻击后微丝虫病的减少与对低分子量肽的识别增强有关。
The effect of vaccination on rates of microfilarial clearance using Dirofilaria immitis in male Lewis rats was examined. Animals were immunized with whole, dead microfilariae or a PBS extract of microfilariae in Freund's adjuvant. The immunized animals, as well as untreated and adjuvant controls, were challenged intravenously with 4 x 10(5) viable microfilariae (mf). The duration of microfilaremia was 15.5 days in rats vaccinated with whole mf, 17.7 days in those vaccinated with a PBS extract, 36.3 days for those vaccinated with adjuvant alone, and greater than 70 days for the untreated group. Analysis of the anti-microfilarial IgG response by ELISA and Western blots demonstrated that immunization induced significant amounts of antibody against high molecular weight peptides, particularly a peptide located at 105 kDa. Antibody levels in both groups of immunized animals continued to rise following challenge, reaching peak levels of 78-80 micrograms/ml on the day of microfilarial clearance. Decreasing microfilaremia following challenge was associated with an enhanced recognition of low molecular weight peptides.