FERMENTATION OF DIETARY FIBER BY HUMAN COLONIC BACTERIA - DISAPPEARANCE OF, SHORT-CHAIN FATTY-ACID PRODUCTION FROM, AND POTENTIAL WATER-HOLDING CAPACITY OF, VARIOUS SUBSTRATES
FERMENTATION OF DIETARY FIBER BY HUMAN COLONIC BACTERIA - DISAPPEARANCE OF, SHORT-CHAIN FATTY-ACID PRODUCTION FROM, AND POTENTIAL WATER-HOLDING CAPACITY OF, VARIOUS SUBSTRATES
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DOI：
10.3109/00365529309096081
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发表时间：
1993-03-01
影响因子：
1.9
通讯作者：
GARLEB, KA
中科院分区：
文献类型：
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作者：
BOURQUIN, LD;TITGEMEYER, EC;GARLEB, KA
使用从三名成年男性受试者中获得的人类结肠细菌在体外发酵几种富含膳食纤维的底物，以评估底物发酵的程度、短链脂肪酸 (SCFA) 的产生以及发酵残留物对粪便量的潜在影响。测试的基质是两种燕麦壳纤维、阿拉伯树胶、羧甲基纤维素 (CMC)、大豆纤维、车前草，以及含有不同比例的燕麦纤维、阿拉伯树胶和 CMC 的六种混合物。除 CMC（816 克）和大豆纤维（778 克）外，所有基质的总膳食纤维含量均超过 900 克/千克。发酵过程中体外有机物的消失量最大的是阿拉伯胶（69.5%），中间的是大豆纤维（56.4%），而两种燕麦纤维（CMC）则低于20%。和车前草。对底物进行平均，产生的乙酸盐、丙酸盐和丁酸盐的摩尔比例为 64:24:12。基质的潜在持水能力（PWHC）是衡量粪便膨胀潜力的指标，CMC 最大（13.5 g H2O/g 基质），阿拉伯树胶（1.92 g）和大豆纤维（1.71 g）最低。混合物的有机物消失和 SCFA 产量与其阿拉伯树胶含量成正比。共混物 PWHC 与 CMC 含量成正比。体外程序可用于预测配制为在体内产生所需效果的纤维混合物的作用。
Several dietary fibre-rich substrates were fermented in vitro with human colonic bacteria obtained from each of three adult male subjects to assess the extent of substrate fermentation, short-chain fatty acid (SCFA) production, and the potential effect of fermented residues on faecal bulk. Substrates tested were two varieties of oat hull fibre, gum arabic, carboxymethylcellulose (CMC), soy fibre, psyllium, and six blends containing oat fibre, gum arabic, and CMC in various proportions. All substrates contained greater than 900 g/kg of total dietary fibre except for CMC (816 g) and soy fibre (778 g). In vitro organic matter disappearance during fermentation was greatest for gum arabic (69.5%), intermediate for soy fibre (56.4%), and less than 20% for the two oat fibres, CMC. and psyllium. Averaged across substrates, acetate, propionate, and butyrate were produced in the molar proportion of 64:24:12. Potential water-holding capacity (PWHC) of substrates, a measure of faecal bulking potential, was greatest for CMC (13.5 g H2O/g substrate) and lowest for gum arabic (1.92 g) and soy fibre (1.71 g). Organic matter disappearance and SCFA production of blends were directly proportional to their gum arabic content. Blend PWHC was proportional to CMC content. In vitro procedures are useful in predicting the actions of fibre blends formulated to produce desirable effects in vivo.