A STUDY OF THE ANTIGENS INVOLVED IN ADENOVIRUS 12 TUMORIGENESIS BY IMMUNODIFFUSION TECHNIQUES.
A STUDY OF THE ANTIGENS INVOLVED IN ADENOVIRUS 12 TUMORIGENESIS BY IMMUNODIFFUSION TECHNIQUES.
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DOI：
10.1084/jem.121.6.955
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发表时间：
1965-06-01
期刊：
影响因子：
--
通讯作者：
ROWE WP
中科院分区：
文献类型：
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作者：
BERMAN LD;ROWE WP
使用琼脂凝胶扩散技术已经确定了在Ad血清中存在三种不同的抗原反应。12只荷瘤仓鼠。这些抗原中只有一种可在肿瘤中直接证实。这种“肿瘤”抗原也在组织培养细胞中感染周期的早期阶段形成。存在于肿瘤中的其他抗原，但只能用含抗体的仓鼠血清间接证明，是经典的类型特异性C抗原，和一种新的抗原，称为D。公元98年。12个肿瘤仓鼠血清，6个在凝胶扩散中与病毒和肿瘤制剂反应，31个仅与肿瘤反应。在凝胶扩散中与病毒抗原反应的血清一致地产生针对病毒和肿瘤抗原的中和抗体和高滴度的CF抗体;然而，许多具有可比抗体滴度的血清在凝胶扩散中不与病毒反应。仅与肿瘤抗原发生凝胶扩散反应的血清也有较高的CF抗体滴度，但与中和抗体无相关性。
The use of the agar gel diffusion technique has established the presence of three distinct antigenic reactions in the sera of Ad. 12 tumor-bearing hamsters. Only one of these antigens is directly demonstrable in the tumor. This "tumor" antigen is also formed during early stages of the infectious cycle in tissue culture cells. Other antigens present in the tumor, but only demonstrable indirectly with the use of antibody-containing serum of tumored hamsters, are the classical type-specific C antigen, and a new antigen, termed D. Of ninety-eight Ad. 12 tumored hamster sera, six reacted in gel diffusion with virus and tumor preparations, and thirty-one with tumor only. Sera which reacted in gel diffusion with viral antigen uniformly bad neutralizing antibody and high titers of CF antibody against viral and tumor antigens; however, many sera with comparable antibody titers did not react with the virus in gel diffusion. Sera which reacted in gel diffusion only with tumor antigen also had high CF antibody titers, but there was no correlation with neutralizing antibody.