Integrated three-dimensional filter separates nanoscale from microscale elements in a microfluidic chip
Integrated three-dimensional filter separates nanoscale from microscale elements in a microfluidic chip
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DOI：
10.1039/c2lc21116e
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Osellame, Roberto
中科院分区：
文献类型：
--
作者：
Amato, Lorenzo;Gu, Yu;Osellame, Roberto
我们报告的集成基于尺寸的三维过滤器，微米大小的孔，在商业微流控芯片。该过滤器是在已经密封的微流体通道内使用双光子聚合的独特能力制造的。这种直接写入技术使得能够通过后处理将滤波器集成到通过标准技术制造的芯片中。过滤器位于两个通道的交叉处，以控制通过过滤器的流量。在罗丹明6G溶液中悬浮3 mm聚苯乙烯球的测试表明，100%的球被阻止，而荧光分子通过过滤器传输。我们演示了长达25分钟的操作，没有任何堵塞的证据。显示了用于从全血中分离血浆的装置的初步验证。此外，过滤器可以通过反向流动来清洁和重复使用。
We report on the integration of a size-based three-dimensional filter, with micrometre-sized pores, in a commercial microfluidic chip. The filter is fabricated inside an already sealed microfluidic channel using the unique capabilities of two-photon polymerization. This direct-write technique enables integration of the filter by post-processing in a chip that has been fabricated by standard technologies. The filter is located at the intersection of two channels in order to control the amount of flow passing through the filter. Tests with a suspension of 3 mm polystyrene spheres in a Rhodamine 6G solution show that 100% of the spheres are stopped, while the fluorescent molecules are transmitted through the filter. We demonstrate operation up to a period of 25 minutes without any evidence of clogging. Preliminary validation of the device for plasma separation from whole blood is shown. Moreover, the filter can be cleaned and reused by reversing the flow.