Immunohistochemical expression of tumor antigens MAGE-A1, MAGE-A3/4, and NY-ESO-1 in cancerous and benign prostatic tissue
Immunohistochemical expression of tumor antigens MAGE-A1, MAGE-A3/4, and NY-ESO-1 in cancerous and benign prostatic tissue
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DOI：
10.1002/pros.20312
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发表时间：
2006-01-01
期刊：
影响因子：
2.8
通讯作者：
Cacic, M
中科院分区：
文献类型：
--
作者：
Hudolin, T;Juretic, A;Cacic, M
目标。为了研究恶性转化或良性增生性前列腺癌组织中MAGE-A和NY-ESO-1/LAGE-1的免疫组织化学表达情况。方法收集1995年至2003年在萨格勒布临床医院泌尿外科门诊就诊的112例前列腺癌患者的前列腺癌组织标本。其中92例前列腺癌标本为根治性前列腺癌，20例良性前列腺增生症标本为经膀胱前列腺切除术。结果MAGE-A1的阳性表达率为10.8%，多MAGE-A和NY-ESO-1/LAGE-1的阳性表达率分别为85.9%和84.8%。免疫组织化学染色仅在胞浆内可见。在同一组织样本中，强阳性区与癌睾丸抗原阴性区共存，可观察到显著的异质性。有趣的是，在57B阳性的病例中，大多数也是D8.38阳性(相关系数r=0.727(P<0.01))。癌睾丸抗原表达与PSA值及Gleason评分均无明显相关性。在良性前列腺增生症组织中，MAGE-A1的阳性表达率为5%，57B和D8.38的阳性表达率为15%，阳性细胞百分率均为10%。结论：前列腺癌组织中肿瘤睾丸抗原的表达具有特殊的相关性，对前列腺癌的诊断和治疗具有潜在意义。
OBJECTIVE. To investigate immunohistochemical expression of MAGE-A and NY-ESO-1/ LAGE-1, cancer testis antigens in prostate tissues showing evidence of malignant transformation or benign hyperplasia.METHODS. 112 prostate samples from patients undergoing surgery at the Urology Clinic at the Zagreb Clinical Hospital Center from 1995 to 2003 were investigated in this study. Of these, 92 carcinoma samples were obtained by radical prostatectomy, and 20 benign prostatic hyperplasia samples by transvesical prostatectomy. Three monoclonal antibodies were used for immunohistochemical staining: 77B for MAGE-Al, 57B for multi-MAGE-A and D8.38 for NY-ESO-1 expression.RESULTS. Expression of MAGE-Al was observed in 10.8% of carcinoma samples, whereas multi-MAGE-A and NY-ESO-1/LAGE-1 stained 85.9% and 84.8% of samples. Immunohistochemical staining was only detectable in the cytoplasm. A significant heterogeneity could be observed within a same tissue sample where areas with strong positivities coexisted with cancer testis antigens negative areas. Interestingly, a majority of 57B positive cases were also found to be D8.38 positive (correlation coefficient r = 0.727 (P < 0.01)). Cancer testis antigens expression was neither significantly correlated with PSA values nor with Gleason score. In benign prostatic hyperplasia tissues MAGE-Al expression was detected in 5%, while 57B and D8.38 staining was observed in 15% samples, and in all cases percentages of positive cells were always < 10%.CONCLUSION. Our data underline the peculiar relevance of cancer testis antigens expression in prostate cancers, with potential implications regarding both diagnosis and therapy.