Compartmental specificity of cellular membrane fusion encoded in SNARE proteins
Compartmental specificity of cellular membrane fusion encoded in SNARE proteins
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DOI：
10.1038/35025000
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发表时间：
2000-09-14
期刊：
影响因子：
64.8
通讯作者：
Rothman, JE
中科院分区：
文献类型：
--
作者：
McNew, JA;Parlati, F;Rothman, JE
有膜囊泡在真核细胞的细胞质的隔室中旅行，通过膜融合将其特定的货物递送到程序化的位置。囊泡v-SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）与靶膜t-SNARE的配对在细胞内膜融合中具有核心作用。我们已经测试了酵母基因组中编码的所有潜在的v-SNARE，通过与标记高尔基体、液泡和质膜的t-SNARE合作来触发融合的能力。在这里，我们发现，在一个显着的程度上，在细胞膜流动的模式是由其分离的SNARE蛋白编码和重演，如SNARE假说所预测的。
Membrane-enveloped vesicles travel among the compartments of the cytoplasm of eukaryotic cells, delivering their specific cargo to programmed locations by membrane fusion. The pairing of vesicle v-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) with target membrane t-SNAREs has a central role in intracellular membrane fusion. We have tested all of the potential v-SNAREs encoded in the yeast genome for their capacity to trigger fusion by partnering with t-SNAREs that mark the Golgi, the vacuole and the plasma membrane. Here we rnd that, to a marked degree, the pattern of membrane flow in the cell is encoded and recapitulated by its isolated SNARE proteins, as predicted by the SNARE hypothesis.