Characterization of carbon nanotube protein corona by using quantitative proteomics
Characterization of carbon nanotube protein corona by using quantitative proteomics
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DOI：
10.1016/j.nano.2012.09.004
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发表时间：
2013-07-01
影响因子：
5.4
通讯作者：
Lam, Yun Wah
中科院分区：
文献类型：
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作者：
Cai, Xiaoning;Ramalingam, Rajkumar;Lam, Yun Wah
纳米材料的蛋白质冠是当材料暴露于体液或细胞内环境时自发且稳定形成的蛋白质的复杂层。在这项研究中，我们利用基于细胞培养中氨基酸的稳定同位素标记（SILAC）定量蛋白质组学来验证人类细胞蛋白质与两种形式的碳纳米颗粒的结合：即多壁碳纳米管（MWCNTs）和炭黑（CB）。测量了超过750种蛋白质与这些材料的相对结合效率。数据表明，多壁碳纳米管和CB结合到非常不同的蛋白质组。研究了蛋白质结合选择性的分子基础。这项研究是第一次大规模表征CNT上的蛋白质冠，为评估CNT作为生物医学工具和新兴污染物的适用性提供了生物化学基础。临床编辑：该研究小组首次对碳纳米管上的蛋白质冠进行了大规模表征，研究了750种蛋白质，并评估了碳纳米管作为生物医学工具和新兴污染物的适用性。(C)2013 Elsevier Inc. All rights reserved.
The protein corona of a nanomaterial is a complex layer of proteins spontaneously and stably formed when the material is exposed to body fluids or intracellular environments. In this study, we utilised stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics to characterise the binding of human cellular proteins to two forms of carbon nanoparticles: namely multi-walled carbon nanotubes (MWCNTs) and carbon black (CB). The relative binding efficiency of over 750 proteins to these materials is measured. The data indicate that MWCNTs and CB bind to vastly different sets of proteins. The molecular basis of selectivity in protein binding is investigated. This study is the first large-scale characterisation of protein corona on CNT, providing the biochemical basis for the assessment of the suitability of CNTs as biomedical tools, and as an emerging pollutant. From the Clinical Editor: This team of investigators performed the first large-scale characterization of protein corona on carbon nanotubes, studying 750 proteins and assessing the suitability of CNTs as biomedical tools and as an emerging pollutant. (C) 2013 Elsevier Inc. All rights reserved.