Survivable stereotaxic surgery in rodents.
Survivable stereotaxic surgery in rodents.
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DOI：
10.3791/880
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发表时间：
2008-10-06
期刊：
影响因子：
--
通讯作者：
Pothos, Emmanuel N
中科院分区：
文献类型：
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作者：
Geiger, Brenda M;Frank, Lauren E;Pothos, Emmanuel N
测量清醒动物脑中神经递质的细胞外基础水平的能力允许确定对CNS的不同全身性挑战（药理学或生理学）的影响。例如，可以直接测量动物的中脑多巴胺投射如何对释放多巴胺的药物（如d-安非他明）或自然刺激（如食物）作出反应。在本视频中，我们将向您展示如何在大鼠大脑中植入针对特定部位的引导套管，如何插入和植入微透析探针，以及如何使用高效液相色谱-电化学检测（HPLC-EC）来测量细胞外可氧化神经递质和代谢物的水平。通过微透析探针局部精确地引入药物允许在化合物的作用机制中对位点特异性进行精细的工作。该技术具有出色的解剖学和化学分辨率，但只有适度的时间分辨率，因为微透析样品通常每20-30分钟处理一次，以确保可检测的神经递质水平。补充的体外工具（即，切片和细胞培养电生理学）可以帮助监测实时神经传递。
The ability to measure extracellular basal levels of neurotransmitters in the brain of awake animals allows for the determination of effects of different systemic challenges (pharmacological or physiological) to the CNS. For example, one can directly measure how the animal's midbrain dopamine projections respond to dopamine-releasing drugs like d-amphetamine or natural stimuli like food. In this video, we show you how to implant guide cannulas targeting specific sites in the rat brain, how to insert and implant a microdialysis probe and how to use high performance liquid chromatography coupled with electrochemical detection (HPLC-EC) to measure extracellular levels of oxidizable neurotransmitters and metabolites. Local precise introduction of drugs through the microdialysis probe allows for refined work on site specificity in a compound s mechanism of action. This technique has excellent anatomical and chemical resolution but only modest time resolution as microdialysis samples are usually processed every 20-30 minutes to ensure detectable neurotransmitter levels. Complementary ex vivo tools (i.e., slice and cell culture electrophysiology) can assist with monitoring real-time neurotransmission.