Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species:: Potential implications for amebic pathogenesis
Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species:: Potential implications for amebic pathogenesis
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DOI：
10.1128/iai.74.1.340-351.2006
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发表时间：
2006-01-01
影响因子：
3.1
通讯作者：
Singh, U
中科院分区：
文献类型：
--
作者：
MacFarlane, RC;Singh, U
溶组织内阿米巴是一种原生动物寄生虫，可引起结肠炎和肝脓肿。已鉴定出几种具有不同毒力水平的内阿米巴属物种和菌株。E. HM-1：IMSS是溶组织E.溶组织阿米巴Rahman是无毒力菌株，而舞帕内阿米巴是无毒力种。我们用了E。由2，110个基因组成的溶组织菌DNA微阵列，以评估这些物种/菌株之间的转录差异，目的是鉴定其表达与毒力表型相关的基因。我们发现有415个基因在大肠杆菌中表达较低。dispar和32个在E. histolyticaRahman比E. histolytica HM-1：IMSS.总的来说，29个基因在两个非毒性种/株中的表达比毒性E. histolytica HM-1：IMSS.有趣的是，一些在应激反应和毒力中具有潜在作用的基因在一种或两种非毒力内阿米巴属物种/菌株中的表达降低。这些基因包括编码铁氢化酶（9.m00419）、过氧化物氧还蛋白（176.m00112）、A型黄素蛋白（6.m00467）、溶菌酶（6.m00454）、鞘磷脂酶C（29.m00231）以及与疟原虫子孢子富苏氨酸-天冬酰胺蛋白（STARP）和链球菌血凝素（238.m00054）同源的假设蛋白质的基因。这些基因在内阿米巴中的功能及其在寄生虫毒力中的具体作用有待确定。我们还发现，一些非长末端重复逆转录转座子（EhLINEs和EhSINEs），这已被证明是调节基因表达和基因组进化，在无毒种/株中的表达低于在大肠杆菌。histolytica HM-1：IMSS.我们的研究结果，确定表达谱和模式指示的毒力表型，可能是有用的毒力的转录框架的特点。
Entamoeba histolytica is a protozoan parasite that causes colitis and liver abscesses. Several Entamoeba species and strains with differing levels of virulence have been identified. E. histolytica HM-1:IMSS is a virulent strain, E. histolytica Rahman is a nonvirulent strain, and Entamoeba dispar is a nonvirulent species. We used an E. histolytica DNA microarray consisting of 2,110 genes to assess the transcriptional differences between these species/strains with the goal of identifying genes whose expression correlated with a virulence phenotype. We found 415 genes expressed at lower levels in E. dispar and 32 genes with lower expression in E. histolytica Rahman than in E. histolytica HM-1:IMSS. Overall, 29 genes had decreased expression in both the nonvirulent species/strains than the virulent E. histolytica HM-1:IMSS. Interestingly, a number of genes with potential roles in stress response and virulence had decreased expression in either one or both nonvirulent Entamoeba species/strains. These included genes encoding Fe hydrogenase (9.m00419), peroxiredoxin (176.m00112), type A flavoprotein (6.m00467), lysozyme (6.m00454), sphingomyelinase C (29.m00231), and a hypothetical protein with homology to both a Plasmodium sporozoite threonine-asparagine-rich protein (STARP) and a streptococcal hemagglutinin (238.m00054). The function of these genes in Entamoeba and their specific roles in parasite virulence need to be determined. We also found that a number of the non-long-terminal-repeat retrotransposons (EhLINEs and EhSINEs), which have been shown to modulate gene expression and genomic evolution, had lower expression in the nonvirulent species/strains than in E. histolytica HM-1:IMSS. Our results, identifying expression profiles and patterns indicative of a virulence phenotype, may be useful in characterizing the transcriptional framework of virulence.