Characterization of constitutive human serum amyloid A protein (SAA4) as an apolipoprotein.
Characterization of constitutive human serum amyloid A protein (SAA4) as an apolipoprotein.
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发表时间：
1995-03
影响因子：
6.5
通讯作者：
M. C. D. Beer;T. Yuan;M. Kindy;B. Asztalos;Paul S. Roheim;F. C. D. Beer
中科院分区：
文献类型：
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作者：
M. C. D. Beer;T. Yuan;M. Kindy;B. Asztalos;Paul S. Roheim;F. C. D. Beer
血清淀粉样蛋白A（serum amyloid A proteins，SAA）是高密度脂蛋白（high density lipoprotein，HDL）的载脂蛋白，是一个同源分子家族.他们可以分为两类。第一组包括充分表征的急性期SAA，其在炎症期间与HDL相关，从而通过置换载脂蛋白（apo）A-I重塑HDL颗粒。第二组包括最近发现的组成型SAA，小鼠SAA 5和人SAA 4。它们作为HDL上的次要载脂蛋白存在，但在稳态期间占总SAA的90%以上。我们已经将人SAA 4表征为载脂蛋白。在体内平衡期间，SAA 4仅在肝脏中合成。已经描述了SAA 4的纯化，并且在26名健康个体中其血浆浓度已经确定为55 +/-13微克/ml。它存在于所有HDL密度等级和极低密度脂蛋白（VLDL）中，但不存在于低密度脂蛋白（LDL）中。使用二维电泳和磷光成像，发现SAA 4与仅三个HDL颗粒的特定亚群相关，不参与细胞的初始胆固醇转移。
Serum amyloid A proteins (SAAs), a family of homologous molecules, are apolipoproteins of high density lipoprotein (HDL). They can be divided into two groups. The first group comprises the well-characterized acute phase SAAs that associate with HDL during inflammation, thereby remodeling the HDL particle by displacing apolipoprotein (apo)A-I. The second group consists of the recently discovered constitutive SAAs, mouse SAA5 and human SAA4. They exist as minor apolipoproteins on HDL but constitute more than 90% of the total SAA during homeostasis. We have characterized human SAA4 as an apolipoprotein. During homeostasis, SAA4 is synthesized only in the liver. Purification of SAA4 has been described and its plasma concentration has been established at 55 +/- 13 micrograms/ml in 26 healthy individuals. It was present on all HDL density classes and very low density lipoprotein (VLDL) but was absent from low density lipoprotein (LDL). Using two-dimensional electrophoresis and phosphorimaging, SAA4 was found to be associated with a specific subpopulation of only three HDL particles, not involved in the initial cholesterol transfer from cells.