Phenotypic and seed structural comparison in hybrids of different Brassica rapa and B. oleracea
Phenotypic and seed structural comparison in hybrids of different Brassica rapa and B. oleracea
复制标题
不同白菜和甘蓝杂交种的表型和种子结构比较
DOI：
10.1111/pbr.12748
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发表时间：
2019
期刊：
影响因子：
2
通讯作者：
Wang Youping
中科院分区：
文献类型：
--
作者：
Rong Hao;Zhu Shuang;Xie Tao;Jiang Jinjin;Wang Youping
甘蓝型油菜是一种历史短、遗传背景窄的重要油料作物。甘蓝与不同白菜型油菜杂交获得了种间杂种。结果表明，在白色花瓣的甘蓝杂种中，黄酮类化合物和酚类化合物的含量降低，这与黄酮类化合物合成基因的表达变化相一致。杂种的种皮与二倍体亲本相似或部分相似，但轮廓清晰。杂种的栅栏层比亲本厚，色素积累量与甘蓝相近，但高于白菜。在杂种中发现了不同大小的蛋白体（PB）。所有杂种的胚根和内子叶均比亲本具有较大但较少的PB。人工合成的甘蓝型油菜外子叶中PB的平均大小也比亲本大，但PB的数量没有显著减少。甘蓝型油菜多倍体化后的表型和种子结构变异对油菜遗传拓宽和育种具有重要意义。
Brassica napusis an important oil species with short history and narrow genetic background. Interspecific hybrids from crosses betweenB. oleraceaand differentB. rapawere obtained. We found the hybrids with white petal resemblingB. oleracea, the flavonoid and phenolic content decreased in hybrids, agreeing with the expressional changes of flavonoid biosynthesis genes. Seed coat of hybrids resembled diploid parents, or partly resembled to each parent with a clear outline. The palisade layer in hybrids was thicker than parents, with similar pigment accumulation asB. oleraceabut more thanB. rapa. Differentially sized protein bodies (PBs) were found in hybrids. The radical and inner cotyledon of all hybrids were identified with larger but less PBs than parents. The average size of PBs in outer cotyledon of resynthesizedB. napuswas also larger than parents, but the number of PBs was not significantly reduced. The phenotypic and seed structural variations after polyploidization ofB. napuswould be interesting for genetic broadening and breeding of rapeseed.