TRIPLE HELIX OF SCHIZOPHYLLUM-COMMUNE POLYSACCHARIDE IN DILUTE-SOLUTION .3. HYDRODYNAMIC PROPERTIES IN WATER
TRIPLE HELIX OF SCHIZOPHYLLUM-COMMUNE POLYSACCHARIDE IN DILUTE-SOLUTION .3. HYDRODYNAMIC PROPERTIES IN WATER
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DOI：
10.1021/ma60078a019
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发表时间：
1980-01-01
期刊：
影响因子：
5.5
通讯作者：
FUJITA, H
中科院分区：
文献类型：
--
作者：
YANAKI, T;NORISUYE, T;FUJITA, H
裂褶菌多糖（裂褶菌多糖）的天然样品及其超声处理的片段（重均分子量（Mv）为105至6 × 106 g mol-1）在25 ℃的水中通过超离心和粘度法进行研究，其中裂褶菌多糖已被发现以三螺旋形式存在。特性粘度[]和极限沉降系数s。的测量显示，在高达Mv= 5 X 105 g mol T1时，该脱叶多糖三螺旋几乎是完全刚性的，但对于更高的Mw获得柔性。在较低分子量范围内的数据通过Yamakawa的理论[]和Yamakawa-Fujii的理论对长刚性圆柱体的s。进行分析，结果是ML（每单位圆柱体长度的摩尔质量）= 2150±150 nm™ 1和d（直径）= 2.6±0.4 nm。该ML给出了每dl，3-D-葡萄糖残基的三股螺旋的螺距值为0.30± 0.02nm，这与所报道的香菇多糖和α-l，3-d-木聚糖在结晶区域中的螺距非常一致。d值为2.6nm，与大叶藻多糖的模型三螺旋直径一致。当ML= 2150±150 nm™ 1，d= 2.6±0.4 nm时，蠕虫状圆柱体的s 0和[]的理论曲线与实验数据符合的余辉长度为200±30 nm。该值表明，阿托茶碱三螺旋比天然胶原蛋白（一种已知的三螺旋生物聚合物）更硬。
A native sample of a Schizophyllum commune polysaccharide (schizophyllan) and its sonicated fragments, ranging in weight-average molecular weight (Mv) from 105 to 6 X 106 g mol™ 1, were studied by ultracentrifugation and viscometry in water at 25 C, in which schizophyllan has been found to exist as a triple helix. Measurements of intrinsic viscosity [] and limiting sedimentation coefficient s0 showed that the schizophyllan triple helix is almost perfectly rigid up to Mv= 5 X 105 g mol™ 1 but acquires flexibility for higher Mw. The data in the lower molecular weight range were analyzed by Yamakawa’s theoryfor [] and Yamakawa-Fujii’s theory for s0 of a long rigid cylinder, with the result that ML (the molar mass per unit cylinder length)= 2150±150 nm™ 1 and d (the diameter)= 2.6±0.4 nm. This ML gives the pitch of the triple helix per dl, 3-D-glucose residue a value of 0.30±0.02 nm, which agrees closely with the reported pitches for lentinan and a ß-l, 3-d-xylan in the crystalline region. The d value 2.6 nm is consistent with the diameter ofthe model triple helix forschizophyllan. With ML= 2150±150 nm™ 1 and d= 2.6±0.4 nm, the persistence length that allows the theoretical curves of s0 and [] for wormlike cylindersto fit thepresent data was found to be 200±30 nm. This value indicates that the schizophyllan triple helix is stiffer than that of native collagen, a known triple-helical biopolymer.