Proteome Mining of Human Follicular Fluid Reveals a Crucial Role of Complement Cascade and Key Biological Pathways in Women Undergoing in Vitro Fertilization
Proteome Mining of Human Follicular Fluid Reveals a Crucial Role of Complement Cascade and Key Biological Pathways in Women Undergoing in Vitro Fertilization
复制标题
DOI：
10.1021/pr900802u
复制
发表时间：
2010-03-01
影响因子：
4.4
通讯作者：
Kovarova, Hana
中科院分区：
文献类型：
--
作者：
Jarkovska, Karla;Martinkova, Jirina;Kovarova, Hana
体外受精(IVF)充满了问题，目前正在尝试蛋白质组学方法来检测卵泡的微环境，以评估可能影响其发育的生物学和免疫学参数。此外，更好地了解生殖过程可能有助于提高每个胚胎移植的试管婴儿出生率，同时避免自发流产或卵巢过度刺激综合征等危及生命的情况。这项研究的主要目的是寻找人类卵泡液(HFF)和血浆中蛋白质组成的具体差异，以确定HFF中积累或不存在的蛋白质。丰富蛋白质的耗尽与多维蛋白质分级相结合，使得对中等和较低丰度蛋白质的研究成为可能。对HFF和成功接受体外受精的妇女的血浆/血清进行配对比较研究，发现蛋白质组成上的重要差异，这可能会提高我们对卵泡微环境及其生物学作用的了解。本研究表明补体的先天免疫功能参与了补体抑制，Perlecan的C末端片段的存在可能与血管生成有关，血管生成是卵泡发生和胎盘发育中的一个重要过程。与凝血有关的蛋白质在滤泡环境中也有差异。观察到了几种特定的蛋白质，其中许多还没有与卵泡/卵母细胞成熟相关。这些蛋白质及其调控途径可能在生殖过程中发挥重要作用。
In vitro fertilization (IVF) is fraught with problems and currently proteomics approaches are being tried out to examine the microenvironment of the follicle in order to assess biological and immunological parameters that may affect its development. Additionally, better understanding of reproductive process may help increase IVF birth rate per embryo transfer and at the same time avoid spontaneous miscarriages or life threatening conditions such as ovarian hyperstimulation syndrome. The primary aim of this study was to search for specific differences in protein composition of human follicular fluid (HFF) and plasma in order to identify proteins that accumulate or are absent in HFF. Depletion of abundant proteins combined with multidimensional protein fractionation allowed the study of middle- and lower-abundance proteins. Paired comparison study examining HFF with plasma/serum from women undergoing successful IVF revealed important differences in the protein composition which may improve our knowledge of the follicular microenvironment and its biological role. This study showed involvement of innate immune function of complement cascade in HFF Complement inhibition and the presence of C-terminal fragment of perlecan suggested possible links to angiogenesis which is a vital process in folliculogenesis and placental development. Differences in proteins associated with blood coagulation were also found in the follicular milieu. Several specific proteins were observed, many of which have not yet been associated with follicle/oocyte maturation. These proteins together with their regulatory pathways may play a vital role in the reproductive process.