Tetrahydrocurcumin exerts protective effect on vincristine induced neuropathy: Behavioral, biochemical, neurophysiological and histological evidence
Tetrahydrocurcumin exerts protective effect on vincristine induced neuropathy: Behavioral, biochemical, neurophysiological and histological evidence
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DOI：
10.1016/j.cbi.2015.06.025
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发表时间：
2015-08-05
影响因子：
5.1
通讯作者：
Balaji, Bhaskar
中科院分区：
文献类型：
--
作者：
Greeshma, N.;Prasanth, K. G.;Balaji, Bhaskar
痛觉过敏、痛觉异常、运动神经传导速度减慢、氧化应激和轴突损伤是化疗所致周围神经病(CIPN)的症状和体征。目前CIPN的治疗/预防策略都是徒劳的，神经病变甚至可能导致停止化疗。本研究观察了四氢姜黄素(THC)40 mg/kg和80 mg/kg对长春新碱诱导的大鼠神经病的保护作用。采用热板(热)法、Randall-Selitto(机械)法评价痛觉过敏，冷板(热)法评价痛觉过敏，坐骨神经功能指数评价功能丧失，福尔马林法评价伤害性反应。进行神经生理学记录，评估运动神经传导速度。测定坐骨神经组织匀浆中总钙水平、氧化应激和肿瘤坏死因子-α水平，以评估神经病变程度。观察坐骨神经组织病理学变化，评价THC对长春新碱的保护作用。本研究以普瑞巴林为标准。大鼠给予THC 80 mg/kg可明显减轻长春新碱所致的神经病理性疼痛，其机制可能与其抗伤害性、抗炎、神经保护、钙抑制和抗氧化作用有关。本研究表明THC可作为化疗药物预防CIPN的一种有前景的候选药物。(C)2015爱思唯尔爱尔兰有限公司。保留所有权利。
Hyperalgesia, allodynia, delayed motor nerve conduction velocity, oxidative stress and axonal damage are signs and symptoms of chemotherapy induced peripheral neuropathy (CIPN). Present treatment/preventive strategies of CIPN are futile and the neuropathy may even lead to discontinuation of chemotherapy. In this study, we evaluated the protective effect of tetrahydrocurcumin (THC) 40 and 80 mg/kg in experimental vincristine induced neuropathy in rats. Hyperalgesia was assessed by hot plate (thermal), Randall-Selitto (mechanical) test, allodynia was assessed by cold plate (thermal) test, functional loss was measured by sciatic function index, nociception was evaluated by formalin test. Neurophysiological recordings were carried out to assess motor nerve conduction velocity. Total calcium levels, oxidative stress and TNF-alpha was measured in sciatic nerve tissue homogenate to assess neuropathy. Histopathological changes was observed on sciatic nerve to assess the protective effect of THC against the vincristine. Pregabalin was used as a standard in this study. Rats administered with THC at 80 mg/kg significantly attenuated the vincristine induced neuropathic pain manifestations which may be due to its multiple actions including anti-nociceptive, anti-inflammatory, neuroprotective, calcium inhibitory and antioxidant effect. This study delineates that THC can be a promising candidate for the prevention of CIPN by chemotherapeutic agents. (C) 2015 Elsevier Ireland Ltd. All rights reserved.