ADHERENCE OF STAPHYLOCOCCI TO INTRAVASCULAR CATHETERS
ADHERENCE OF STAPHYLOCOCCI TO INTRAVASCULAR CATHETERS
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DOI：
10.1099/00222615-28-4-249
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发表时间：
1989-04-01
影响因子：
3
通讯作者：
KRISTINSSON, KG
中科院分区：
文献类型：
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作者：
KRISTINSSON, KG
对7株表皮葡萄球菌和3株链球菌的粘附性进行了研究。用ATP生物发光法、超声处理后培养法和扫描电镜法检测金黄色葡萄球菌对三种类型血管内导管的敏感性。研究的导管材料为硅橡胶弹性体、热塑性聚氨酯和Hydromer涂层聚氨酯，Hydromer涂层可吸收水分并在导管周围提供亲水鞘。在37 ℃的旋转振荡器上，在磷酸盐缓冲盐水中进行粘附测定。C，导管预涂血清和未涂血清，结果与细菌疏水性相关。细菌粘附存在广泛的菌株间差异; aureus和产黏菌S. epidermidis菌株比非粘液产生菌株粘附得更好。总体而言，Hydromer涂层导管的细菌粘附少于聚氨酯和硅胶导管，但与细菌疏水性无关。导管的血清涂层导致细菌粘附显著减少。
Adherence of seven strains of Staphylococcus epidermidis and three strains of S. aureus to three types of intravascular catheters was assessed by ATP bioluminescence, by culture after ultrasonication and by scanning electronmicroscopy. The catheter materials studied were silicone elastomer, thermoplastic polyurethane and polyurethane coated with Hydromer, a coating which absorbs water and provides a hydrophilic sheath around the catheter. The adherence assays were performed in phosphate-buffered saline on a rotary shaker at 37.degree. C, with the catheters precoated with serum and uncoated, and the results were correlated with bacterial hydrophobicity. There was wide strain-to-strain variation in bacterial adherence; S. aureus and slime-producing S. epidermidis strains adhered better than did non-slime-producing strains. Overall, there was less bacterial adherence to Hydromer-coated catheters than to polyurethane and silicone catheters but it was unrelated to bacterial hydrophobicity. Serum coating of catheters resulted in marked reduction of bacterial adherence.