Spreading Depolarization during the Acute Stage of Experimental Subarachnoid Hemorrhage in Mice.
Spreading Depolarization during the Acute Stage of Experimental Subarachnoid Hemorrhage in Mice.
复制标题
小鼠实验性蛛网膜下腔出血急性期的去极化扩散。
DOI：
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
O. Sakowitz
中科院分区：
文献类型：
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作者：
Zelong Zheng;M. Schoell;R. Sánchez;C. Diehl;A. Unterberg;O. Sakowitz
扩散去极化（SD）被认为是蛛网膜下腔出血（SAH）后迟发性脑缺血的病理机制。然而，SD 在 SAH 急性期的作用仍不清楚。本研究的目的是调查 (a) 通过内在光信号 (IOS) 成像发生 SD，(b) 氯胺酮对 SD 的影响，以及 (c) 在小鼠实验性 SAH 急性期期间产生的脑水肿（脑含水量 (BWC)）。 SAH是通过血管内丝穿孔法引发的。 SAH 或假手术后，每半小时给予氯胺酮或盐水 30 mg/kg。使用 IOS 记录组织光反射率的变化。 BWC 在急性期进行测量。总体而言，SAH 组出现 199 个 SD，假手术组出现 33 个 SD。这些 SD 显示出不同的起源和传播模式。与生理盐水相比，氯胺酮减少了 SD 的扩散，并影响了 SD 的幅度、持续时间和速度。然而，氯胺酮并不能阻止SD的发生。此外，氯胺酮不会降低 SAH 后的 BWC。这些结果表明，SAH 急性期 SD 发生率较高。 SD 在发生、起源和传播方面具有异质性。目前尚不清楚氯胺酮对 SD 的影响是否对 SAH 后有治疗作用。
Spreading depolarization (SD) has been suggested as a pathomechanism for delayed cerebral ischemia after subarachnoid hemorrhage (SAH). However, the role of SD during the acute phase of SAH is still unclear. The objective of this study was to investigate (a) the occurrence of SD with intrinsic optical signal (IOS) imaging, (b) the effect of ketamine on SD, and (c) the resulting brain edema (brain water content (BWC)) during the acute stage of experimental SAH in mice. SAH was elicited by the endovascular filament perforation method. After SAH or sham operation, ketamine or saline, 30 mg/kg, was given every half hour. Changes in tissue light reflectance were recorded with IOS. BWC was measured during the acute stage. Overall, 199 SDs occurred in SAH groups and 33 SDs appeared in sham groups. These SDs displayed distinct originating and spreading patterns. Compared with saline, ketamine decreased SD spread and influenced the amplitude, duration, and speed of SD. However, the occurrence of SD was not prevented by ketamine. Moreover, ketamine did not reduce BWC after SAH. These results demonstrate that SD occurs with a high incidence during the acute stage of SAH. SDs are heterogeneous in incidence, origination, and propagation. It remains unclear whether ketamine effects on SD may be viewed as therapeutically beneficial after SAH.
影响因子：
2.5
作者：
Ba, AM;Guiou, M;Toga, AW
通讯作者：
Toga, AW