Combination of stromal vascular fraction and Ad‑COMP‑Ang1 gene therapy improves long‑term therapeutic efficacy for diabetes‑induced erectile dysfunction
Combination of stromal vascular fraction and Ad‑COMP‑Ang1 gene therapy improves long‑term therapeutic efficacy for diabetes‑induced erectile dysfunction
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基质血管部分和 Ad-COMP-Ang1 基因疗法的组合可提高糖尿病引起的勃起功能障碍的长期治疗效果
DOI：
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发表时间：
2018
影响因子：
2.9
通讯作者：
Hai‑Rong Jin
中科院分区：
文献类型：
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作者：
Guo-Nan Yin;Lin Wang;Xiang-Nan Lin;Lei Shi;Zhen-Lin Gao;Feng-Chan Han;Ping Li;Yin-Chuan Jin;Jun‑Kyu Suh;Ji‑Kan Ryu;Xiong Wang;Hai‑Rong Jin
糖尿病勃起功能障碍(ED)患者对目前可用的口服磷酸二酯酶-5抑制剂反应较差。因此，需要对糖尿病勃起功能障碍进行治疗。基质血管成分(SVF)和腺病毒介导的软骨。寡聚基质血管生成素-1(Ad-comp-Ang1)基因在阴茎勃起中起关键作用。我们以前报道过，SVF和Ad-Comp-Ang1在恢复勃起功能方面只有短期效果。需要进一步改进ED疗法，以获得持久的效果。在本研究中，我们旨在测试SVF和Ad-Comp-Ang1的联合应用是否能延长糖尿病ED的勃起效应。我们发现，联合疗法通过促进阴茎内皮细胞和神经细胞的再生，在恢复勃起功能方面显示出长期的效果。SVF和Ad-comp-Ang1联合治疗可显著恢复糖尿病小鼠海绵状内皮细胞数、周细胞数、内皮细胞-细胞连接，降低海绵状内皮细胞通透性，促进神经再生，至少持续4周。综上所述，这是对SVF和Ad-comp-Ang1联合治疗通过对内皮细胞和神经细胞再生的双重作用恢复勃起功能的长期效果的初步描述。这种联合疗法对糖尿病ED的治疗可能具有治疗潜力。
Men with diabetic erectile dysfunction (ED) respond poorly to the currently available oral phosphodiesterase‑5 inhibitors..Therefore, functional therapies for diabetic ED are needed. Stromal vascular fraction (SVF) and the adenovirus‑mediated cartilage.oligomeric matrix angiopoietin‑1 (Ad‑COMP‑Ang1) gene are known to play critical roles in penile erection. We previously reported.that SVF and Ad‑COMP‑Ang1 have only a short‑term effect in restoring erectile function. Further improvements to ED therapy.are needed for long‑lasting effects. In the present study, we aimed to test if the combination of SVF and Ad‑COMP‑Ang1 could.extend the erection effect in diabetic ED. We found that the combination therapy showed a long‑term effect in restoring erectile.function through enhanced penile endothelial and neural cell regeneration. Combination therapy with SVF and Ad‑COMP‑Ang1.notably restored cavernous endothelial cell numbers, pericyte numbers, endothelial cell–cell junctions, decreased cavernous.endothelial cell permeability, and promoted neural regeneration for at least 4 weeks in diabetic mice. In summary, this is an.initial description of the long‑term effect of combination therapy with SVF and Ad‑COMP‑Ang1 in restoring erectile function.through a dual effect on endothelial and neural cell regeneration. Such combination therapy may have therapeutic potential.for the treatment of diabetic ED.