Characterization of Synergy between Cucumber mosaic virus and Tobacco necrosis virus in Nicotiana benthamiana
Characterization of Synergy between Cucumber mosaic virus and Tobacco necrosis virus in Nicotiana benthamiana
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DOI：
10.1111/j.1439-0434.2007.01279.x
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发表时间：
2007-09
影响因子：
1.5
通讯作者：
D. Xi;H. Feng;L. Lan;J. Du;Jian-Hui Wang;Zhong-wei Zhang;L. Xue;W. Xu;Hong-hui Lin
中科院分区：
文献类型：
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作者：
D. Xi;H. Feng;L. Lan;J. Du;Jian-Hui Wang;Zhong-wei Zhang;L. Xue;W. Xu;Hong-hui Lin
黄瓜花叶病毒（CMV）和烟草坏死病毒（TNV）混合侵染本氏烟（Nicotiana benthamiana）表现出协同作用，导致症状加重。混合感染的植株中CMV（+）RNA和衣壳蛋白（CP）的积累量显著高于单感染植株。在双重感染的植株中，TNV（+）RNA和CP水平也略有增加。CMV和TNV协同侵染引起的丙二醛和过氧化氢（H2O2）含量的增加和过氧化氢酶活性的下降幅度大于单独侵染。过氧化物酶和超氧化物歧化酶的活性迅速增加，在第一个10天接种后（dpi）在双感染的植物，然后下降，而酶活性继续增加后10 dpi在单感染的植物和有较高的酶活性在后期比共感染的植物。这些结果表明，CMV和TNV的协同感染在N.本塞姆氏植物和两种病毒之间的协同作用是相互的。
Mixed infections of Nicotiana benthamiana plants by Cucumber mosaic virus (CMV) and Tobacco necrosis virus (TNV) exhibit a synergistic interaction and result in symptom enhancement. Accumulation of CMV(+) RNA as well as capsid protein (CP) in mixed infection was considerably higher than that of singly-infected plants. There was also a slight increase in TNV(+) RNA and CP levels in doubly infected plants. Synergistic infection by CMV- and TNV-induced higher increase in the levels of malonyldialdehyde, hydrogen peroxide (H 2 O 2 ) and more decline in the activities of catalase than singly infected ones. Both peroxidase and superoxide dismutase activities increased rapidly for the first 10 days post inoculation (dpi) in doubly-infected plants and then declined, whereas the enzyme activities continued to increase after 10 dpi in singly infected plants and had higher enzyme activities in the late stages than that of co-infected plants. These results suggest that synergistic infection by CMV and TNV produced severes oxidative stress in N. benthamiana plants and the synergy between the two viruses was mutual.